Natural compounds derived from foods modulate nitric oxide production and oxidative status in epithelial lung cells

Maayan Zaslaver; Sarit Offer; Zohar Kerem; Aliza H. Stark; Joel Ira Weller; Avraham Eliraz; Zecharia Madar

doi:10.1021/jf052000u

Natural compounds derived from foods modulate nitric oxide production and oxidative status in epithelial lung cells

Maayan Zaslaver, Sarit Offer, Zohar Kerem, Aliza H. Stark, Joel Ira Weller, Avraham Eliraz, Zecharia Madar^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The effects of natural antioxidants on nitric oxide (NO) modulation and oxidative status were determined in rat epithelial lung cells (L-2). Cells were stimulated with cytokines and treated with one of the following: resveratrol, soybean saponin group B (SSB), quercetin, genistein, olive leaf polyphenol concentrate (OLPC), or N-acetyl-L-cystein (NAC). NAC had no effect on NO levels, whereas resveratrol and OLPC were found to be effective in reducing nitrite levels, modifying iNOS mRNA, and decreasing free radical production. OLPC affected the levels of MnSOD while resveratrol did not, indicating that they act via different pathways. Quercetin and genistein reduced nitrite levels without affecting iNOS levels, presumably by scavenging NO. SSB did not affect nitrite levels, but exposure did reduce iNOS mRNA expression and protein levels, possibly due to antioxidant activity. Naturally occuring antioxidants, in particular resveratrol and OLPC, may have therapeutic potential in the treatment of inflammatory diseases.

Original language	American English
Pages (from-to)	9934-9939
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	53
Issue number	26
DOIs	https://doi.org/10.1021/jf052000u
State	Published - 28 Dec 2005

Keywords

Cytokines
Inducible nitric oxide synthase
Lung epithelial cells
Nitric oxide
Polyphenols
Resveratrol

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10.1021/jf052000u

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title = "Natural compounds derived from foods modulate nitric oxide production and oxidative status in epithelial lung cells",

abstract = "The effects of natural antioxidants on nitric oxide (NO) modulation and oxidative status were determined in rat epithelial lung cells (L-2). Cells were stimulated with cytokines and treated with one of the following: resveratrol, soybean saponin group B (SSB), quercetin, genistein, olive leaf polyphenol concentrate (OLPC), or N-acetyl-L-cystein (NAC). NAC had no effect on NO levels, whereas resveratrol and OLPC were found to be effective in reducing nitrite levels, modifying iNOS mRNA, and decreasing free radical production. OLPC affected the levels of MnSOD while resveratrol did not, indicating that they act via different pathways. Quercetin and genistein reduced nitrite levels without affecting iNOS levels, presumably by scavenging NO. SSB did not affect nitrite levels, but exposure did reduce iNOS mRNA expression and protein levels, possibly due to antioxidant activity. Naturally occuring antioxidants, in particular resveratrol and OLPC, may have therapeutic potential in the treatment of inflammatory diseases.",

keywords = "Cytokines, Inducible nitric oxide synthase, Lung epithelial cells, Nitric oxide, Polyphenols, Resveratrol",

author = "Maayan Zaslaver and Sarit Offer and Zohar Kerem and Stark, {Aliza H.} and Weller, {Joel Ira} and Avraham Eliraz and Zecharia Madar",

year = "2005",

month = dec,

day = "28",

doi = "10.1021/jf052000u",

language = "American English",

volume = "53",

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T1 - Natural compounds derived from foods modulate nitric oxide production and oxidative status in epithelial lung cells

AU - Zaslaver, Maayan

AU - Offer, Sarit

AU - Kerem, Zohar

AU - Stark, Aliza H.

AU - Weller, Joel Ira

AU - Eliraz, Avraham

AU - Madar, Zecharia

PY - 2005/12/28

Y1 - 2005/12/28

N2 - The effects of natural antioxidants on nitric oxide (NO) modulation and oxidative status were determined in rat epithelial lung cells (L-2). Cells were stimulated with cytokines and treated with one of the following: resveratrol, soybean saponin group B (SSB), quercetin, genistein, olive leaf polyphenol concentrate (OLPC), or N-acetyl-L-cystein (NAC). NAC had no effect on NO levels, whereas resveratrol and OLPC were found to be effective in reducing nitrite levels, modifying iNOS mRNA, and decreasing free radical production. OLPC affected the levels of MnSOD while resveratrol did not, indicating that they act via different pathways. Quercetin and genistein reduced nitrite levels without affecting iNOS levels, presumably by scavenging NO. SSB did not affect nitrite levels, but exposure did reduce iNOS mRNA expression and protein levels, possibly due to antioxidant activity. Naturally occuring antioxidants, in particular resveratrol and OLPC, may have therapeutic potential in the treatment of inflammatory diseases.

AB - The effects of natural antioxidants on nitric oxide (NO) modulation and oxidative status were determined in rat epithelial lung cells (L-2). Cells were stimulated with cytokines and treated with one of the following: resveratrol, soybean saponin group B (SSB), quercetin, genistein, olive leaf polyphenol concentrate (OLPC), or N-acetyl-L-cystein (NAC). NAC had no effect on NO levels, whereas resveratrol and OLPC were found to be effective in reducing nitrite levels, modifying iNOS mRNA, and decreasing free radical production. OLPC affected the levels of MnSOD while resveratrol did not, indicating that they act via different pathways. Quercetin and genistein reduced nitrite levels without affecting iNOS levels, presumably by scavenging NO. SSB did not affect nitrite levels, but exposure did reduce iNOS mRNA expression and protein levels, possibly due to antioxidant activity. Naturally occuring antioxidants, in particular resveratrol and OLPC, may have therapeutic potential in the treatment of inflammatory diseases.

KW - Cytokines

KW - Inducible nitric oxide synthase

KW - Lung epithelial cells

KW - Nitric oxide

KW - Polyphenols

KW - Resveratrol

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DO - 10.1021/jf052000u

M3 - Article

C2 - 16366677

AN - SCOPUS:30544431731

SN - 0021-8561

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SP - 9934

EP - 9939

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 26

ER -

Natural compounds derived from foods modulate nitric oxide production and oxidative status in epithelial lung cells

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Keywords

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