NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation

Antonija Miletic; Maja Lenartic; Branka Popovic; Ilija Brizic; Tihana Trsan; Karmela Miklic; Ofer Mandelboim; Astrid Krmpotic; Stipan Jonjic

doi:10.1002/eji.201646763

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation

Antonija Miletic
, Maja Lenartic
, Branka Popovic
, Ilija Brizic
, Tihana Trsan
, Karmela Miklic
, Ofer Mandelboim
, Astrid Krmpotic
, Stipan Jonjic^*

^*Corresponding author for this work

Department of Immunology and Cancer Research

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

NKp46/NCR1 is an activating NK-cell receptor implicated in the control of various viral and bacterial infections. Recent findings also suggest that it plays a role in shaping the adaptive immune response to pathogens. Using NCR1-deficient (NCR1^gfp/gfp) mice, we provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection (MCMV). The absence of NCR1 resulted in impaired maturation, function and NK-cell migration to regional lymph nodes. In addition, CD4⁺ T-cell activation and follicular helper T-cell (Tfh) generation were reduced, leading to inferior germinal center (GC) B-cell maturation. As a consequence, NCR1^gfp/gfp mice produced lower amounts of MCMV-specific antibodies upon infection, which correlated with lower number of virus-specific antibody secreting cells in analyzed lymph nodes.

Original language	English
Pages (from-to)	1443-1456
Number of pages	14
Journal	European Journal of Immunology
Volume	47
Issue number	9
DOIs	https://doi.org/10.1002/eji.201646763
State	Published - Sep 2017

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antiviral antibodies
Follicular helper T cells
Germinal center B cells
NCR1 receptor
NK-cell immunoregulation
Viral infection

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10.1002/eji.201646763

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title = "NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation",

abstract = "NKp46/NCR1 is an activating NK-cell receptor implicated in the control of various viral and bacterial infections. Recent findings also suggest that it plays a role in shaping the adaptive immune response to pathogens. Using NCR1-deficient (NCR1gfp/gfp) mice, we provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection (MCMV). The absence of NCR1 resulted in impaired maturation, function and NK-cell migration to regional lymph nodes. In addition, CD4+ T-cell activation and follicular helper T-cell (Tfh) generation were reduced, leading to inferior germinal center (GC) B-cell maturation. As a consequence, NCR1gfp/gfp mice produced lower amounts of MCMV-specific antibodies upon infection, which correlated with lower number of virus-specific antibody secreting cells in analyzed lymph nodes.",

keywords = "Antiviral antibodies, Follicular helper T cells, Germinal center B cells, NCR1 receptor, NK-cell immunoregulation, Viral infection",

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year = "2017",

month = sep,

doi = "10.1002/eji.201646763",

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AU - Miletic, Antonija

AU - Lenartic, Maja

AU - Popovic, Branka

AU - Brizic, Ilija

AU - Trsan, Tihana

AU - Miklic, Karmela

AU - Mandelboim, Ofer

AU - Krmpotic, Astrid

AU - Jonjic, Stipan

PY - 2017/9

Y1 - 2017/9

N2 - NKp46/NCR1 is an activating NK-cell receptor implicated in the control of various viral and bacterial infections. Recent findings also suggest that it plays a role in shaping the adaptive immune response to pathogens. Using NCR1-deficient (NCR1gfp/gfp) mice, we provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection (MCMV). The absence of NCR1 resulted in impaired maturation, function and NK-cell migration to regional lymph nodes. In addition, CD4+ T-cell activation and follicular helper T-cell (Tfh) generation were reduced, leading to inferior germinal center (GC) B-cell maturation. As a consequence, NCR1gfp/gfp mice produced lower amounts of MCMV-specific antibodies upon infection, which correlated with lower number of virus-specific antibody secreting cells in analyzed lymph nodes.

AB - NKp46/NCR1 is an activating NK-cell receptor implicated in the control of various viral and bacterial infections. Recent findings also suggest that it plays a role in shaping the adaptive immune response to pathogens. Using NCR1-deficient (NCR1gfp/gfp) mice, we provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection (MCMV). The absence of NCR1 resulted in impaired maturation, function and NK-cell migration to regional lymph nodes. In addition, CD4+ T-cell activation and follicular helper T-cell (Tfh) generation were reduced, leading to inferior germinal center (GC) B-cell maturation. As a consequence, NCR1gfp/gfp mice produced lower amounts of MCMV-specific antibodies upon infection, which correlated with lower number of virus-specific antibody secreting cells in analyzed lymph nodes.

KW - Antiviral antibodies

KW - Follicular helper T cells

KW - Germinal center B cells

KW - NCR1 receptor

KW - NK-cell immunoregulation

KW - Viral infection

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JF - European Journal of Immunology

IS - 9

ER -

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation

Abstract

Bibliographical note

UN SDGs

Keywords

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