Near-field optical imaging of unstained bacteria: Comparison with normal atomic force and far-field optical microscopy in air and aqueous media

Nily Ben-Ami, Anna Radko, Udi Ben-Ami, Klony Lieberman, Zvi Rothman, Itzhak Rabin, Aaron Lewis*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

10 Scopus citations

Abstract

Simultaneous near-field scanning optical and atomic force imaging of bacteria is presented. The bacteria imaged in these studies were unstained. The near-field optical images had excellent signal-to-noise and showed excellent contrast even in these unstained specimens. The images obtained were interpreted in terms of the images that have been obtained by transmission electron microscopy and X-ray imaging. The results show that bacterial near-field optical imaging is going to be a very important tool in the arsenal of the bacteriologist both in terms of understanding the fundamental processes in the life cycle of bacteria with and without cytochemical staining and in terms of clinical diagnostic applications.

Original languageEnglish
Pages (from-to)321-325
Number of pages5
JournalUltramicroscopy
Volume71
Issue number1-4
DOIs
StatePublished - 1 Mar 1998
EventProceedings of the 1997 4th International Conference on Near-Field Optics and Related Techniques, NFO-4 - Jerusalem, Israel
Duration: 9 Feb 199713 Feb 1997

Fingerprint

Dive into the research topics of 'Near-field optical imaging of unstained bacteria: Comparison with normal atomic force and far-field optical microscopy in air and aqueous media'. Together they form a unique fingerprint.

Cite this