Neonatal blood is more resistant to oxidative stress induced by stable nitroxide radicals than adult blood

Tali Silberstein, David Mankuta, Alexander I. Shames, Gertz I. Likhtenshtein, Dan Meyerstein, Naomi Meyerstein, Oshra Saphier*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Neonate erythrocytes are more susceptible to oxidizing drugs than adults; however, there are controversial reports in the literature regarding the total antioxidant capacity of neonate blood. Stable nitroxide radicals (NRs) are reduced by blood and some other biological materials to the corresponding hydroxylamines. The kinetics of the nitroxide's disappearance using electron paramagnetic resonance (EPR) spectroscopy, provides useful biochemical and biophysical information about the antioxidant properties of biological systems. In order to investigate the antioxidant properties in the newborn's blood, we applied this novel method on 38 umbilical vein blood samples and 40 healthy adults. The NR, 5-Dimethylaminonaphthalene-1-sulfonyl-4-amino-2,2,6,6,- tetramethyl-piperidine-oxyl (R), was used for this purpose. Ascorbate is the only known natural antioxidant that reduces R. We found that the reduction rates of R in neonate's whole blood are significantly higher (P < 0.001) than the reduction rates of R in adult's whole blood. However, there were no significant differences in the antioxidant capacity between the two groups. Newborn's blood has significantly higher ability to deal with oxidative stress, caused by R, in comparison with adult blood. We suggest that the system that responds to the recycling of ascorbate is more efficient in neonate blood than in adult's blood.

Original languageAmerican English
Pages (from-to)233-237
Number of pages5
JournalArchives of Gynecology and Obstetrics
Issue number3
StatePublished - Mar 2008
Externally publishedYes


Dive into the research topics of 'Neonatal blood is more resistant to oxidative stress induced by stable nitroxide radicals than adult blood'. Together they form a unique fingerprint.

Cite this