Nerve growth factor-induced angiogenesis: 2. The quail chorioallantoic membrane assay

Philip Lazarovici; Adi Lahiani; Galit Gincberg; Dikla Haham; Cezary Marcinkiewicz; Peter I. Lelkes

doi:10.1007/978-1-4939-7571-6_19

Nerve growth factor-induced angiogenesis: 2. The quail chorioallantoic membrane assay

Philip Lazarovici^*, Adi Lahiani, Galit Gincberg, Dikla Haham, Cezary Marcinkiewicz, Peter I. Lelkes

^*Corresponding author for this work

School of Pharmacy

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Scopus citations

Abstract

The avian chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development. Therefore, the models of chicken and quail assays represent robust experimental platforms to study angiogenesis, which reflects perturbation of the entire vascular tree. This experimental approach, when combined with fractal morphometry, is sensitive to changes in vascular branching pattern and density. Nerve growth factor is a neurotrophin promoting angiogenesis in CAM models. Here, we provide a detailed protocol of the quail CAM, shell-less model, to study nerve growth factor effects on blood capillary sprouting. The quail CAM assay may be beneficial in investigations of cellular and molecular aspects of neurotrophin-induced angiogenesis and for developing novel anti-angiogenesis and anticancer therapies.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	251-259
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-4939-7571-6_19
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1727
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC 2018.

Keywords

Analysis
Angiogenesis
Avian
Chorioallantoic membrane assay
Fractal morphometry
Nerve growth factor
Vascular branching and density

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-7571-6_19

Cite this

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abstract = "The avian chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development. Therefore, the models of chicken and quail assays represent robust experimental platforms to study angiogenesis, which reflects perturbation of the entire vascular tree. This experimental approach, when combined with fractal morphometry, is sensitive to changes in vascular branching pattern and density. Nerve growth factor is a neurotrophin promoting angiogenesis in CAM models. Here, we provide a detailed protocol of the quail CAM, shell-less model, to study nerve growth factor effects on blood capillary sprouting. The quail CAM assay may be beneficial in investigations of cellular and molecular aspects of neurotrophin-induced angiogenesis and for developing novel anti-angiogenesis and anticancer therapies.",

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author = "Philip Lazarovici and Adi Lahiani and Galit Gincberg and Dikla Haham and Cezary Marcinkiewicz and Lelkes, {Peter I.}",

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AU - Lazarovici, Philip

AU - Lahiani, Adi

AU - Gincberg, Galit

AU - Haham, Dikla

AU - Marcinkiewicz, Cezary

AU - Lelkes, Peter I.

N1 - Publisher Copyright: © Springer Science+Business Media, LLC 2018.

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KW - Angiogenesis

KW - Avian

KW - Chorioallantoic membrane assay

KW - Fractal morphometry

KW - Nerve growth factor

KW - Vascular branching and density

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Nerve growth factor-induced angiogenesis: 2. The quail chorioallantoic membrane assay

Abstract

Publication series

Bibliographical note

Keywords

UN SDGs

Access to Document

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