Abstract
Neural stem cells (NSCs) are self-renewing multipotent cells that line the neural-tube and generate all the nervous system. Understanding NSC biology is fundamental for neurodevelopmental research and therapy. Many studies emphasized the need to culture NSCs, which are typically purified from mammalian embryonic/adult brains. These sources are somewhat limited in terms of quantity, availability and animal ethical guidelines. Therefore, new sources are needed. The chick is a powerful system for experimental embryology which contributed enormously to neurodevelopmental concepts. Its accessibility, genetic/molecular manipulations, and homology to other vertebrates, makes it valuable for developmental biology research. Recently, we identified a population of NSCs in the chick hindbrain. It resides in rhombomere-boundaries, expresses Sox2 and generates progenitors and neurons. Here, we investigated whether these cells can recapitulate hindbrain development in culture. By developing approaches to propagate and image cells, manipulate their growth-conditions and separate them into subpopulations, we demonstrate the ordered formation of multipotent and self-renewing neurospheres that maintain regional identity and display differential stem/differentiation/proliferation properties. Live imaging revealed new cellular dynamics in the culture. Collectively, these NSC cultures reproduce major aspects of hindbrain development in-vitro, proposing the chick as a model for culturing hindbrain-NSCs that can be directly applied to other neural-tube domains and species.
Original language | American English |
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Article number | 13920 |
Journal | Scientific Reports |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - 1 Dec 2018 |
Bibliographical note
Funding Information:This work was supported by The Israel Science Foundation (DSD, grant number 1515/16), The Chief Scientist Office of the Ministry of Health, Israel (DSD, grant number 3-0000-15441), United State-Israel Binational Science Foundation (DSD, grant number 2015087) and Lady Davis Fellowship Trust (YP). We wish to thank Dr. T. Burstyn-Cohen for providing reagents and for her insights and discussions.
Publisher Copyright:
© 2018, The Author(s).