TY - JOUR
T1 - Neuroprotective effects of gangliosides on pardaxin-induced toxicity in PC12 cells
AU - Ellren-Kashi, Osnat
AU - Trembovler, Victoria
AU - Abu-Raya, Saleh
AU - Lazarovici, Philip
AU - Shohami, Esther
PY - 1997/10
Y1 - 1997/10
N2 - Pardaxin-induced cytotoxicity in PC12 cells represents a model system for the study of mechanisms underlying neuronal degeneration, as well as a tool for the evaluation of potential neuroprotective agents. In the present investigation, viability of PC12 cells following exposure to pardaxin (1-20 μM), was assessed in parallel to the determination of PGE2 content in the culture medium. A series of gangliosides (GM1, GM2, GM3, GD1a, and GQ1b) were found to have a protective effect on pardaxin-induced cytotoxicity and to inhibit the production of PGE2. In contrast, the neurotrophins NGF and FGF, and the Ca-channel blocker nifedipine, had no effect on survival or on PGE2 production. Inhibitors of PGE2 release did not improve survival, suggesting that the apparent association between PX-induced PGE2 release and cytotoxicity are independent events.
AB - Pardaxin-induced cytotoxicity in PC12 cells represents a model system for the study of mechanisms underlying neuronal degeneration, as well as a tool for the evaluation of potential neuroprotective agents. In the present investigation, viability of PC12 cells following exposure to pardaxin (1-20 μM), was assessed in parallel to the determination of PGE2 content in the culture medium. A series of gangliosides (GM1, GM2, GM3, GD1a, and GQ1b) were found to have a protective effect on pardaxin-induced cytotoxicity and to inhibit the production of PGE2. In contrast, the neurotrophins NGF and FGF, and the Ca-channel blocker nifedipine, had no effect on survival or on PGE2 production. Inhibitors of PGE2 release did not improve survival, suggesting that the apparent association between PX-induced PGE2 release and cytotoxicity are independent events.
UR - http://www.scopus.com/inward/record.url?scp=0030778216&partnerID=8YFLogxK
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AN - SCOPUS:0030778216
SN - 1058-8108
VL - 6
SP - 285
EP - 292
JO - Journal of Natural Toxins
JF - Journal of Natural Toxins
IS - 3
ER -