Neuroprotective effects of gangliosides on pardaxin-induced toxicity in PC12 cells

Osnat Ellren-Kashi, Victoria Trembovler, Saleh Abu-Raya, Philip Lazarovici, Esther Shohami*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Pardaxin-induced cytotoxicity in PC12 cells represents a model system for the study of mechanisms underlying neuronal degeneration, as well as a tool for the evaluation of potential neuroprotective agents. In the present investigation, viability of PC12 cells following exposure to pardaxin (1-20 μM), was assessed in parallel to the determination of PGE2 content in the culture medium. A series of gangliosides (GM1, GM2, GM3, GD1a, and GQ1b) were found to have a protective effect on pardaxin-induced cytotoxicity and to inhibit the production of PGE2. In contrast, the neurotrophins NGF and FGF, and the Ca-channel blocker nifedipine, had no effect on survival or on PGE2 production. Inhibitors of PGE2 release did not improve survival, suggesting that the apparent association between PX-induced PGE2 release and cytotoxicity are independent events.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalJournal of Natural Toxins
Volume6
Issue number3
StatePublished - Oct 1997

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