Neuroprotective effects of nimodipine and nifedipine in the NGF-differentiated PC12 cells exposed to oxygen-glucose deprivation or trophic withdrawal

Shimon Lecht*, Elena Rotfeld, Hadar Arien-Zakay, Rinat Tabakman, Henry Matzner, Rami Yaka, Peter I. Lelkes, Philip Lazarovici

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The goal of this study was to compare the neuroprotective properties of the l-type Ca2+ channel blockers, nimodipine and nifedipine, using nerve growth factor (NGF)-differentiated PC12 neuronal cultures exposed to oxygen-glucose deprivation (OGD) and trophic withdrawal-induced cell death. Nimodipine (1-100μM) conferred 65±13% neuroprotection upon exposure to OGD and 35±6% neuroprotection towards different trophic withdrawal-induced cell death measured by lactate dehydrogenase and caspase 3 activities. The time window of nimodipine conferred neuroprotection was detected during the first 5h but not at longer OGD exposures. Nifedipine (1-100μM), to a lower potency than nimodipine, conferred 30-55±8% neuroprotection towards OGD in PC12 cells and 29±5% in rat hypocampal slices, and 10±3% neuroprotection at 100μM towards trophic withdrawal-induced PC12 cell death. The ability to demonstrate that nimodipine conferred neuroprotection in a narrow therapeutic time-window indicates that the OGD PC12 model mimics the in vivo models and therefore suitable for neuroprotective drug discovery and development.

Original languageAmerican English
Pages (from-to)465-469
Number of pages5
JournalInternational Journal of Developmental Neuroscience
Volume30
Issue number6
DOIs
StatePublished - Oct 2012

Keywords

  • Hypocampal slices
  • Neuroprotection
  • Nifedipine
  • Nimodipine
  • Oxygen and glucose deprivation
  • PC12

Fingerprint

Dive into the research topics of 'Neuroprotective effects of nimodipine and nifedipine in the NGF-differentiated PC12 cells exposed to oxygen-glucose deprivation or trophic withdrawal'. Together they form a unique fingerprint.

Cite this