TY - JOUR
T1 - Neutral and acid proteinases from free living nematode Turbatrix aceti. Partial purification and characterization
AU - Gertler, A.
AU - Madar, Z.
AU - Haas, Y.
PY - 1979
Y1 - 1979
N2 - 1. 1. Neutral and acid proteinases from Turbatrix aceti were isolated and partially purified, 400-500-fold and characterized. 2. 2. Neutral proteinase is a thiol proteinase with a molecular weight of 22000 and pH optimum of 6.5. It exhibits chymotrypsin-like specificity and is inhibited by p-mercuribenzoate and β-mercaptoethanol. 3. 3. Neutral proteinase is also inhibited by a specific irreversible inhibitor of serine proteinases-diisopropylphosphofluoridate with second-order rate constant of inactivation = 21 min-1 M-1. However, it is not effected by high molecular weight inhibitors of serine proteinases. 4. 4. Acid proteinase is a typical carboxyl proteinase with a molecular weight of 60,000 and pH optimum of 3.5. It is inhibited by a specific inhibitor of carboxyl proteinases 1,2-epoxy-3-(p-nitrophenoxy) propane.
AB - 1. 1. Neutral and acid proteinases from Turbatrix aceti were isolated and partially purified, 400-500-fold and characterized. 2. 2. Neutral proteinase is a thiol proteinase with a molecular weight of 22000 and pH optimum of 6.5. It exhibits chymotrypsin-like specificity and is inhibited by p-mercuribenzoate and β-mercaptoethanol. 3. 3. Neutral proteinase is also inhibited by a specific irreversible inhibitor of serine proteinases-diisopropylphosphofluoridate with second-order rate constant of inactivation = 21 min-1 M-1. However, it is not effected by high molecular weight inhibitors of serine proteinases. 4. 4. Acid proteinase is a typical carboxyl proteinase with a molecular weight of 60,000 and pH optimum of 3.5. It is inhibited by a specific inhibitor of carboxyl proteinases 1,2-epoxy-3-(p-nitrophenoxy) propane.
UR - http://www.scopus.com/inward/record.url?scp=49249149567&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(79)90282-7
DO - 10.1016/0305-0491(79)90282-7
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AN - SCOPUS:49249149567
SN - 0305-0491
VL - 64
SP - 357
EP - 362
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 4
ER -