New insights into Ice growth and melting modifications by antifreeze proteins

Maya Bar-Dolev, Yeliz Celik, J. S. Wettlaufer, Peter L. Davies, Ido Braslavsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

Antifreeze proteins (AFPs) evolved in many organisms, allowing them to survive in cold climates by controlling ice crystal growth. The specific interactions of AFPs with ice determine their potential applications in agriculture, food preservation and medicine. AFPs control the shapes of ice crystals in a manner characteristic of the particular AFP type. Moderately active AFPs cause the formation of elongated bipyramidal crystals, often with seemingly defined facets, while hyperactive AFPs produce more varied crystal shapes. These differentmorphologies are generally considered to be growth shapes. In a series of bright light and fluorescent microscopy observations of ice crystals in solutions containing different AFPs, we show that crystal shaping also occurs during melting. In particular, the characteristic ice shapes observed in solutions ofmost hyperactiveAFPs are formed during melting.We relate these findings to the affinities of the hyperactive AFPs for the basal plane of ice. Our results demonstrate the relation between basal plane affinity and hyperactivity and show a clear difference in the ice-shaping mechanisms of most moderate and hyperactive AFPs. This study provides key aspects associated with the identification of hyperactive AFPs.

Original languageAmerican English
Pages (from-to)3249-3259
Number of pages11
JournalJournal of the Royal Society Interface
Volume9
Issue number77
DOIs
StatePublished - 7 Dec 2012

Keywords

  • Antifreeze proteins
  • Crystal growth
  • Hyperactive antifreeze proteins
  • Ice-binding proteins
  • Ice-structuring proteins
  • Melting shapes

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