New sequencing methodologies reveal interplay between multiple RNA-binding proteins and their RNAs

Sahar Melamed*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

12 Scopus citations

Abstract

It is now established that base-pairing regulatory RNAs are key players in post-transcriptional regulatory networks where they affect the translation and/or stability of their target RNAs. In many cases, the base-pairing between two RNAs is facilitated by an RNA-binding protein (RBP) that serves as an RNA chaperone. Recent advances in sequencing methods have revealed the RNA populations bound by the RBPs, yielding insights valuable into regulatory networks. Further analyses of these networks can improve our understanding of the roles played by RBPs in the regulation of gene expression by regulatory RNAs, especially when multiple RBPs are involved. For example, using an RNA sequencing-based methodology that captures RNA–RNA pairs on RBP, an interplay between two RBPs in bacteria that compete on the same RNA–RNA pair was revealed. In this case, one protein promotes negative regulation of the target RNA, while the second protein can block this regulation. In this mini-review, I outline the exciting future directions that can be taken to deepen our understanding of the roles played by RBPs in post-transcriptional regulation, and discuss how the different sequencing methods can assist in deciphering the relationships among RBPs, and between the RBPs and the RNAs they bind. Having a more detailed picture of the RBPs–RNAs network will elucidate how bacteria can have nuanced control of gene expression, critical for survival in the varied environments in which bacteria live.

Original languageAmerican English
Pages (from-to)713-717
Number of pages5
JournalCurrent Genetics
Volume66
Issue number4
DOIs
StatePublished - 1 Aug 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020, This is a U.S. government work and its text is not subject to copyright protection in the United States; however, its text may be subject to foreign copyright protection.

Keywords

  • Hfq
  • ProQ
  • RIL-seq
  • RNA chaperones
  • RNA-binding proteins
  • Small RNAs

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