Nitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1

Yoram Barak; Merav Yarmus; Roni Shapira; Jaap Van Rijn

doi:10.1016/S0378-1097(02)00454-8

Nitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1

Yoram Barak
, Merav Yarmus
, Roni Shapira
, Jaap Van Rijn^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Two relatively low-copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid-cured cells resulted in a significant intermediate nitrite accumulation as compared to wild-type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0-kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8-kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters.

Original language	English
Pages (from-to)	111-116
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	208
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(02)00454-8
State	Published - 19 Feb 2002

Bibliographical note

Funding Information:
The study was supported by an intramural grant established by the Swiss Friends of the Hebrew University of Jerusalem.

Keywords

Nitrite reduction
Paracoccus
Plasmid

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10.1016/S0378-1097(02)00454-8

Cite this

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title = "Nitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1",

abstract = "Two relatively low-copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid-cured cells resulted in a significant intermediate nitrite accumulation as compared to wild-type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0-kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8-kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters.",

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author = "Yoram Barak and Merav Yarmus and Roni Shapira and \{Van Rijn\}, Jaap",

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AU - Yarmus, Merav

AU - Shapira, Roni

AU - Van Rijn, Jaap

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AB - Two relatively low-copy plasmids of 9 and 16 kb were found to comprise the extrachromosomal DNA of a Paracoccus strain. Reduction of nitrate by plasmid-cured cells resulted in a significant intermediate nitrite accumulation as compared to wild-type cells. By examining nitrate reduction by transformants containing one of the two plasmids, it was found that nitrite accumulation was influenced by the 9.0-kb plasmid, designated as pYR1. Subcloning analysis showed that a 1.8-kb fragment of this plasmid affected nitrite accumulation. Sequence analysis of this fragment revealed the presence of five open reading frames. One of the six deduced proteins showed a strong homology to ABC transporters.

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KW - Plasmid

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Nitrite reduction in Paracoccus sp. is affected by a novel plasmid pYR1

Abstract

Bibliographical note

Keywords

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