TY - JOUR
T1 - Non-specific esterases in mites-a comparative study
AU - Capua, Shlomo
AU - Cohen, Ephraim
AU - Gerson, Uri
PY - 1990
Y1 - 1990
N2 - 1. A variety of non-specific cytosolic and solubilized microsomal esterase isozymes from three mite species Rhizoglyphus robini, Tetranychus urticae and Phytoseiulus persimilis, representing three different acarine suborders, were separated by polyacrylamide electrophoresis. 2. The relevance of enzyme heterogeneity with respect to habitats and tolerance to pesticides of these mites is discussed. 3. The kinetics of the separated esterase isozymes, which were measured directly on the acrylamide gel, revealed a diversity of intra- and interspecific Km values. 4. Most of the esterase isozymes present in the cytosolic and microsomal fractions were sensitive to the organophosphorus compound diisopropyi fluorophosphate. 5. They were less inhibited by the carbamate eserine and by p-hydroxymercuribenzoate. 6. The enzymes were highly sensitive to transition state analogs represented by several trifluoroketones.
AB - 1. A variety of non-specific cytosolic and solubilized microsomal esterase isozymes from three mite species Rhizoglyphus robini, Tetranychus urticae and Phytoseiulus persimilis, representing three different acarine suborders, were separated by polyacrylamide electrophoresis. 2. The relevance of enzyme heterogeneity with respect to habitats and tolerance to pesticides of these mites is discussed. 3. The kinetics of the separated esterase isozymes, which were measured directly on the acrylamide gel, revealed a diversity of intra- and interspecific Km values. 4. Most of the esterase isozymes present in the cytosolic and microsomal fractions were sensitive to the organophosphorus compound diisopropyi fluorophosphate. 5. They were less inhibited by the carbamate eserine and by p-hydroxymercuribenzoate. 6. The enzymes were highly sensitive to transition state analogs represented by several trifluoroketones.
UR - http://www.scopus.com/inward/record.url?scp=0025120598&partnerID=8YFLogxK
U2 - 10.1016/0742-8413(90)90056-F
DO - 10.1016/0742-8413(90)90056-F
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AN - SCOPUS:0025120598
SN - 0306-4492
VL - 96
SP - 125
EP - 130
JO - Comparative Biochemistry and Physiology, Part C
JF - Comparative Biochemistry and Physiology, Part C
IS - 1
ER -