Non-specific esterases in mites-a comparative study

Shlomo Capua, Ephraim Cohen*, Uri Gerson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

1. A variety of non-specific cytosolic and solubilized microsomal esterase isozymes from three mite species Rhizoglyphus robini, Tetranychus urticae and Phytoseiulus persimilis, representing three different acarine suborders, were separated by polyacrylamide electrophoresis. 2. The relevance of enzyme heterogeneity with respect to habitats and tolerance to pesticides of these mites is discussed. 3. The kinetics of the separated esterase isozymes, which were measured directly on the acrylamide gel, revealed a diversity of intra- and interspecific Km values. 4. Most of the esterase isozymes present in the cytosolic and microsomal fractions were sensitive to the organophosphorus compound diisopropyi fluorophosphate. 5. They were less inhibited by the carbamate eserine and by p-hydroxymercuribenzoate. 6. The enzymes were highly sensitive to transition state analogs represented by several trifluoroketones.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalComparative Biochemistry and Physiology, Part C
Volume96
Issue number1
DOIs
StatePublished - 1990

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