TY - JOUR
T1 - Nonconventional involvement of LysRS in the molecular mechanism of USF2 transcriptional activity in FcεRI-activated mast cells
AU - Lee, Yu Nee
AU - Razin, Ehud
PY - 2005/10
Y1 - 2005/10
N2 - Reports of the biological multifunctional activity of various aminoacyl tRNA synthetases have recently accumulated in the literature. The primary function of these critical enzymes is to charge various tRNAs with their appropriate amino acids, thus producing the building blocks of protein synthesis. We have previously shown that lysyl tRNA synthetase (LysRS) associates with microphthalmia transcription factor (MITF) and regulates its activity by synthesis of Ap4A in mast cells. Here, we show for the first time that LysRS associates with another transcription factor, USF2, which unlike MITF, is ubiquitously expressed in eukaryotic cells. Using mast cells, we have found that USF2 is negatively regulated by Hint and Ap4A acts as a positive regulator of USF2 by a molecular mechanism similar to that described for MITF. Since USF2 plays a significant role in a variety of cellular functions, our finding suggests that LysRS and Ap4A may be involved in general regulation of gene transcription.
AB - Reports of the biological multifunctional activity of various aminoacyl tRNA synthetases have recently accumulated in the literature. The primary function of these critical enzymes is to charge various tRNAs with their appropriate amino acids, thus producing the building blocks of protein synthesis. We have previously shown that lysyl tRNA synthetase (LysRS) associates with microphthalmia transcription factor (MITF) and regulates its activity by synthesis of Ap4A in mast cells. Here, we show for the first time that LysRS associates with another transcription factor, USF2, which unlike MITF, is ubiquitously expressed in eukaryotic cells. Using mast cells, we have found that USF2 is negatively regulated by Hint and Ap4A acts as a positive regulator of USF2 by a molecular mechanism similar to that described for MITF. Since USF2 plays a significant role in a variety of cellular functions, our finding suggests that LysRS and Ap4A may be involved in general regulation of gene transcription.
UR - http://www.scopus.com/inward/record.url?scp=26444461483&partnerID=8YFLogxK
U2 - 10.1128/MCB.25.20.8904-8912.2005
DO - 10.1128/MCB.25.20.8904-8912.2005
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C2 - 16199869
AN - SCOPUS:26444461483
SN - 0270-7306
VL - 25
SP - 8904
EP - 8912
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 20
ER -