TY - JOUR
T1 - Nonimmunogenic radiation induced lymphoma immunity induction by a somatic cell hybrid
AU - Yefenof, Eitan
AU - Goldapfel, Mira
AU - Ber, Rosalie
PY - 1982/5
Y1 - 1982/5
N2 - The cell line designated PIR-2 is a nonimmunogenic X ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo, Fusion of PIR-2 with an allogeneic "universal fuser', A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonim munogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionabl.
AB - The cell line designated PIR-2 is a nonimmunogenic X ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo, Fusion of PIR-2 with an allogeneic "universal fuser', A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonim munogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionabl.
UR - http://www.scopus.com/inward/record.url?scp=0019965917&partnerID=8YFLogxK
U2 - 10.1093/jnci/68.5.841
DO - 10.1093/jnci/68.5.841
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C2 - 6951093
AN - SCOPUS:0019965917
SN - 0027-8874
VL - 68
SP - 841
EP - 849
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -