Noninvasive nonlinear focusing and imaging through strongly scattering turbid layers

Ori Katz, Eran Small, Yefeng Guan, Yaron Silberberg*

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

153 Scopus citations


Diffraction-limited imaging through complex scattering media is a long-sought-after goal with important applications in biomedical research. In recent years, highresolution wavefront shaping has emerged as a powerful approach to generate a sharp focus through highly scattering, visually opaque samples. However, it requires a localized feedback signal from the target point of interest, which necessitates an invasive procedure in all-optical techniques. Here, we show that by exploiting optical nonlinearities, a diffraction-limited focus can be formed inside or through a complex sample, even when the feedback signal is not localized. We prove our approach theoretically and numerically, and experimentally demonstrate it with a two-photon fluorescence signal through highly scattering biological samples. We use the formed focus to perform two-photon microscopy through highly scattering, visually opaque layers.

Original languageAmerican English
Pages (from-to)170-174
Number of pages5
Issue number3
StatePublished - 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Optical Society of America.


  • Microscopy
  • Multiple scattering
  • Nonlinear microscopy
  • Speckle
  • Turbid media


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