Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites

Gadi Peleg, Aaron Lewis*, Michal Linial, Leslie M. Loew

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Membrane potential around single molecules has been measured by using the nonlinear optical phenomenon of second harmonic generation. This advance results from the interaction between a highly dipolar molecule with a selectively directed highly polarizable 1-nm gold particle. With this approach, a second harmonic signal, which is enhanced by the nanoparticle, is detected from a volume of nanometric dimensions. This present work clearly shows that functional cellular imaging around single molecules is possible by selectively directing an antibody with a 1-nm gold label to a specific membrane protein. The results of this work open the way for three- dimensional, high resolution functional imaging of membrane electrophysiology in cells and cellular networks.

Original languageAmerican English
Pages (from-to)6700-6704
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number12
DOIs
StatePublished - 8 Jun 1999

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