Abstract
The dansyl ester of anandamide was prepared and showed intense fluorescence on silica gel thin-layer chromatography when viewed under long-wavelength ultraviolet light (detection limit, 15 fmol). A high-performance liquid chromatography method for the quantitation of anandamide was developed using a C18 column (250x4.6 mm) with gradient elution (1% acetic acid-methanol) and detection at 255 nm. The method was applied to the measurement of anandamide in media from cultured hepatocytes. Sample preparation involved extraction with a C18 cartridge, derivatization with dansyl chloride, thin-layer chromatography, and quantitation. The detection limit in hepatocyte media is 4.3 nmol at a signal-to-noise ratio of three.
Original language | English |
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Pages (from-to) | 93-99 |
Number of pages | 7 |
Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
Volume | 740 |
Issue number | 1 |
DOIs | |
State | Published - 31 Mar 2000 |
Keywords
- Anandamide
- Derivatization, LC