Novel and sensitive method for the detection of anandamide by the use of its dansyl derivative

Boris Yagen, Sumner Burstein

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The dansyl ester of anandamide was prepared and showed intense fluorescence on silica gel thin-layer chromatography when viewed under long-wavelength ultraviolet light (detection limit, 15 fmol). A high-performance liquid chromatography method for the quantitation of anandamide was developed using a C18 column (250x4.6 mm) with gradient elution (1% acetic acid-methanol) and detection at 255 nm. The method was applied to the measurement of anandamide in media from cultured hepatocytes. Sample preparation involved extraction with a C18 cartridge, derivatization with dansyl chloride, thin-layer chromatography, and quantitation. The detection limit in hepatocyte media is 4.3 nmol at a signal-to-noise ratio of three.

Original languageEnglish
Pages (from-to)93-99
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume740
Issue number1
DOIs
StatePublished - 31 Mar 2000

Keywords

  • Anandamide
  • Derivatization, LC

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