Novel methodology for enzymatic removal of atrazine from water by CBD- fusion protein immobilized on cellulose

Carl Kauffmann; Oded Shoseyov; Etai Shpigel; Edward A. Bayer; Raphael Lamed; Yuval Shoham; Raphi T. Mandelbaum

doi:10.1021/es990754h

Novel methodology for enzymatic removal of atrazine from water by CBD- fusion protein immobilized on cellulose

Carl Kauffmann, Oded Shoseyov^*, Etai Shpigel, Edward A. Bayer, Raphael Lamed, Yuval Shoham, Raphi T. Mandelbaum

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

s-Triazines including atrazine are heavily used agricultural herbicides, and their extensive removal from industrial wastewater is required before these effluents can be disposed. The use of enzymes for this purpose is an important potential alternative to conventional methods of detoxification. The purpose of the present study was to develop an enzyme-based technology for the treatment of atrazine contaminated water. In this paper we describe the construction and expression of two fusion proteins which dechlorinate atrazine while being firmly bound to an insoluble cellulose matrix. Dechlorination of atrazine produces in one enzymatic step the nonregulated compound hydroxyatrazine from the regulated mother compound, atrazine.

Original language	English
Pages (from-to)	1292-1296
Number of pages	5
Journal	Environmental Science and Technology
Volume	34
Issue number	7
DOIs	https://doi.org/10.1021/es990754h
State	Published - 1 Apr 2000

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abstract = "s-Triazines including atrazine are heavily used agricultural herbicides, and their extensive removal from industrial wastewater is required before these effluents can be disposed. The use of enzymes for this purpose is an important potential alternative to conventional methods of detoxification. The purpose of the present study was to develop an enzyme-based technology for the treatment of atrazine contaminated water. In this paper we describe the construction and expression of two fusion proteins which dechlorinate atrazine while being firmly bound to an insoluble cellulose matrix. Dechlorination of atrazine produces in one enzymatic step the nonregulated compound hydroxyatrazine from the regulated mother compound, atrazine.",

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AU - Kauffmann, Carl

AU - Shoseyov, Oded

AU - Shpigel, Etai

AU - Bayer, Edward A.

AU - Lamed, Raphael

AU - Shoham, Yuval

AU - Mandelbaum, Raphi T.

PY - 2000/4/1

Y1 - 2000/4/1

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AB - s-Triazines including atrazine are heavily used agricultural herbicides, and their extensive removal from industrial wastewater is required before these effluents can be disposed. The use of enzymes for this purpose is an important potential alternative to conventional methods of detoxification. The purpose of the present study was to develop an enzyme-based technology for the treatment of atrazine contaminated water. In this paper we describe the construction and expression of two fusion proteins which dechlorinate atrazine while being firmly bound to an insoluble cellulose matrix. Dechlorination of atrazine produces in one enzymatic step the nonregulated compound hydroxyatrazine from the regulated mother compound, atrazine.

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Novel methodology for enzymatic removal of atrazine from water by CBD- fusion protein immobilized on cellulose

Abstract

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