Abstract
All of the different cells within an individual share the same genome, whereas epigenetic variations underlie the plasticity in gene expression from the stem cells that differentiate during development, repair, and aging. Beyond differences in covalent and noncovalent modifications of chromatin between different cell types, there are also lineage-relevant variations in the structure proteins of the nuclear envelope called lamins that contribute to chromatin organization and even transcription factor localization. Here we focus on imaging these various molecular factors with the relevant optical microscopy in the context of multiple methods for physically manipulating nuclei.
Original language | English |
---|---|
Title of host publication | Handbook of Imaging in Biological Mechanics |
Publisher | CRC Press |
Pages | 401-413 |
Number of pages | 13 |
ISBN (Electronic) | 9781466588141 |
ISBN (Print) | 9781466588134 |
DOIs | |
State | Published - 1 Jan 2014 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015 by Taylor & Francis Group, LLC.