TY - JOUR
T1 - Nucleic acid-functionalized Pt nanoparticles
T2 - Catalytic labels for the amplified electrochemical detection of biomolecules
AU - Polsky, Ronen
AU - Gill, Ron
AU - Kaganovsky, Lubov
AU - Willner, Itamar
PY - 2006/4/1
Y1 - 2006/4/1
N2 - Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 × 10-11 M). Similarly, the association of aptamer- functionalized Pt-NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 × 10-9 M.
AB - Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 × 10-11 M). Similarly, the association of aptamer- functionalized Pt-NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 × 10-9 M.
UR - http://www.scopus.com/inward/record.url?scp=33645695504&partnerID=8YFLogxK
U2 - 10.1021/ac0519864
DO - 10.1021/ac0519864
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C2 - 16579607
AN - SCOPUS:33645695504
SN - 0003-2700
VL - 78
SP - 2268
EP - 2271
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 7
ER -