Objective-type dark-field illumination for scattering from microbeads

Ido Braslavsky; Roee Amit; B. M.Jaffar Ali; Opher Gileadi; Amos Oppenheim; Joel Stavans

doi:10.1364/AO.40.005650

Objective-type dark-field illumination for scattering from microbeads

Ido Braslavsky, Roee Amit, B. M.Jaffar Ali, Opher Gileadi, Amos Oppenheim, Joel Stavans

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-tonoise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method’s use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.

Original language	English
Pages (from-to)	5650-56579
Number of pages	50930
Journal	Applied Optics
Volume	40
Issue number	31
DOIs	https://doi.org/10.1364/AO.40.005650
State	Published - 1 Nov 2001
Externally published	Yes

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title = "Objective-type dark-field illumination for scattering from microbeads",

abstract = "We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-tonoise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method{\textquoteright}s use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.",

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AU - Braslavsky, Ido

AU - Amit, Roee

AU - Ali, B. M.Jaffar

AU - Gileadi, Opher

AU - Oppenheim, Amos

AU - Stavans, Joel

PY - 2001/11/1

Y1 - 2001/11/1

N2 - We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-tonoise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method’s use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.

AB - We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the backreflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-tonoise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method’s use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.

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Objective-type dark-field illumination for scattering from microbeads

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