A hyperpolarised-NMR acquisition approach that is sensitive to the process of glucose-6-phosphate anomerization is presented. Using selective depolarisation of one of the anomer's signals, it is possible to observe the replenishing of this signal due to the fast anomeric exchange of this compound. The forward to reverse reaction rate constants ratio was ca. 1.6.
Bibliographical noteFunding Information:
The authors thank Dr Talia Harris for her assistance in the course of this study. This project has received funding from the European Union's Horizon 2020 Research and Innovation Program under grant agreement no. 667192, and from the Israel Innovation Authority, KAMIN Incentive program, grant agreement no. 63361.
© The Royal Society of Chemistry.