Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR

Sivaranjan Uppala; Ayelet Gamliel; Gal Sapir; Jacob Sosna; J. Moshe Gomori; Rachel Katz-Brull

doi:10.1039/d0ra08022e

Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR

Sivaranjan Uppala, Ayelet Gamliel, Gal Sapir, Jacob Sosna, J. Moshe Gomori, Rachel Katz-Brull^*

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

A hyperpolarised-NMR acquisition approach that is sensitive to the process of glucose-6-phosphate anomerization is presented. Using selective depolarisation of one of the anomer's signals, it is possible to observe the replenishing of this signal due to the fast anomeric exchange of this compound. The forward to reverse reaction rate constants ratio was ca. 1.6.

Original language	English
Pages (from-to)	41197-41201
Number of pages	5
Journal	RSC Advances
Volume	10
Issue number	67
DOIs	https://doi.org/10.1039/d0ra08022e
State	Published - 11 Nov 2020

Bibliographical note

Funding Information:
The authors thank Dr Talia Harris for her assistance in the course of this study. This project has received funding from the European Union's Horizon 2020 Research and Innovation Program under grant agreement no. 667192, and from the Israel Innovation Authority, KAMIN Incentive program, grant agreement no. 63361.

Publisher Copyright:
© The Royal Society of Chemistry.

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AU - Sosna, Jacob

AU - Gomori, J. Moshe

AU - Katz-Brull, Rachel

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Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR

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