TY - JOUR
T1 - Occurrence of polyamines in coliphages T5, phiX174 and in phage infected bacteria
AU - Bachrach, U.
AU - Fischer, R.
AU - Klein, I.
PY - 1975
Y1 - 1975
N2 - The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin layer chromatography and mass spectrometry of dansyl derivatives, as well as by ion exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.
AB - The polyamine spermidine and the diamine putrescine have been detected in coliphages T5 and phiX174. Polyamines were identified by thin layer chromatography and mass spectrometry of dansyl derivatives, as well as by ion exchange chromatography. In phiX174 phages, polyamines were sufficient to neutralize 0.5% of DNA phosphates. The polyamine content of T5 phages depended on growth media and purification procedures, but at least 1% of DNA phosphates were neutralized by polyamines. After infection, an increase in cellular polyamine was noticed. This increase paralleled variations in ornithine decarboxylase activity.
UR - http://www.scopus.com/inward/record.url?scp=0016631664&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-26-3-287
DO - 10.1099/0022-1317-26-3-287
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C2 - 1091718
AN - SCOPUS:0016631664
SN - 0022-1317
VL - 26
SP - 287
EP - 294
JO - Journal of General Virology
JF - Journal of General Virology
IS - 3
ER -