TY - JOUR
T1 - Oceanicola antarcticus sp. nov. and Oceanicola flagellatus sp. nov., moderately halophilic bacteria isolated from seawater
AU - Huo, Ying Yi
AU - Li, Zheng Yang
AU - You, Hong
AU - Wang, Chun Sheng
AU - Post, Anton F.
AU - Oren, Aharon
AU - Xu, Xue Wei
N1 - Publisher Copyright:
© 2014 IUMS.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Two Gram-stain-negative, aerobic, moderately halophilic, rod-shaped bacteria (strains Ar-45T and DY470T) were isolated from seawater collected from the Southern Ocean and the Pacific Ocean, respectively. Growth of strain Ar-45T was observed with between 0.5 and 10.0% (w/v) NaCl (optimally with 0.5–3.0%) and between pH 5.5 and 9.5. Strain DY470T grew in the presence of 0.5–7.5% (w/v) NaCl (optimally with 2.0%) and at pH 5.5–8.5. Chemotaxonomic analysis showed Q-10 as the respiratory quinone for both strains. The major fatty acids (.5%) of strain Ar-45T were C16: 0, C19: 0 cyclo ɷ8c and C18: 1ɷ7c, while those of strain DY470T were C18: 1ɷ7c, C16: 0 and 11-methyl C18: 1ɷ7c. The DNA G+C contents of the two strains were 62.0 and 61.8 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains Ar-45T and DY470T were related most closely to the genus Oceanicola, with sequence similarities of 97.4–94.0 and 97.7–94.7%, respectively. The DNA–DNA hybridization value between strain Ar-45T and Oceanicola marinus LMG 23705T was 22.0%. Levels of DNA-DNA relatedness between strain DY470T and Oceanicola nitratireducens LMG 24663T and Oceanicola batsensis DSM 15984T were 32.5 and 26.1%, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, strains Ar-45T and DY470T are considered to represent two novel species of the genus Oceanicola, for which the names Oceanicola antarcticus (type strain Ar-45T=CGMCC 1.12662T=LMG 27868T) and Oceanicola flagellatus (type strain DY470T=CGMCC 1.12664T=LMG 27871T) are proposed.
AB - Two Gram-stain-negative, aerobic, moderately halophilic, rod-shaped bacteria (strains Ar-45T and DY470T) were isolated from seawater collected from the Southern Ocean and the Pacific Ocean, respectively. Growth of strain Ar-45T was observed with between 0.5 and 10.0% (w/v) NaCl (optimally with 0.5–3.0%) and between pH 5.5 and 9.5. Strain DY470T grew in the presence of 0.5–7.5% (w/v) NaCl (optimally with 2.0%) and at pH 5.5–8.5. Chemotaxonomic analysis showed Q-10 as the respiratory quinone for both strains. The major fatty acids (.5%) of strain Ar-45T were C16: 0, C19: 0 cyclo ɷ8c and C18: 1ɷ7c, while those of strain DY470T were C18: 1ɷ7c, C16: 0 and 11-methyl C18: 1ɷ7c. The DNA G+C contents of the two strains were 62.0 and 61.8 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains Ar-45T and DY470T were related most closely to the genus Oceanicola, with sequence similarities of 97.4–94.0 and 97.7–94.7%, respectively. The DNA–DNA hybridization value between strain Ar-45T and Oceanicola marinus LMG 23705T was 22.0%. Levels of DNA-DNA relatedness between strain DY470T and Oceanicola nitratireducens LMG 24663T and Oceanicola batsensis DSM 15984T were 32.5 and 26.1%, respectively. Based on phylogenetic, chemotaxonomic and phenotypic data, strains Ar-45T and DY470T are considered to represent two novel species of the genus Oceanicola, for which the names Oceanicola antarcticus (type strain Ar-45T=CGMCC 1.12662T=LMG 27868T) and Oceanicola flagellatus (type strain DY470T=CGMCC 1.12664T=LMG 27871T) are proposed.
UR - http://www.scopus.com/inward/record.url?scp=84907048389&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.062588-0
DO - 10.1099/ijs.0.062588-0
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 24899659
AN - SCOPUS:84907048389
SN - 1466-5026
VL - 64
SP - 2975
EP - 2979
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
ER -