TY - JOUR
T1 - One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices
AU - Bavli, Danny
AU - Ezra, Elishai
AU - Kitsberg, Daniel
AU - Vosk-Artzi, Margarita
AU - Murthy, Shashi K.
AU - Nahmias, Yaakov
N1 - Publisher Copyright:
© 2016 AIP Publishing LLC.
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotinlabeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α- Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.
AB - Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotinlabeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α- Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.
UR - http://www.scopus.com/inward/record.url?scp=84961584087&partnerID=8YFLogxK
U2 - 10.1063/1.4944741
DO - 10.1063/1.4944741
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AN - SCOPUS:84961584087
SN - 1932-1058
VL - 10
JO - Biomicrofluidics
JF - Biomicrofluidics
IS - 2
M1 - 024112
ER -