One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices

Danny Bavli, Elishai Ezra, Daniel Kitsberg, Margarita Vosk-Artzi, Shashi K. Murthy, Yaakov Nahmias*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotinlabeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α- Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.

Original languageAmerican English
Article number024112
JournalBiomicrofluidics
Volume10
Issue number2
DOIs
StatePublished - 1 Mar 2016

Bibliographical note

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© 2016 AIP Publishing LLC.

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