Opiates and cultured neuroblastoma x glioma cells. Effect of cyclic AMP and polyamine levels and on ornithine decarboxylase and protein kinase activities

D. Benalal, U. Bachrach

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

In cultured NG 108-15 neuroblastoma x glioma cells, opiates decreased cellular cyclic AMP and polyamine levels. This decrease was related to the inhibition of ornithine decarboxylase and cyclic AMP-dependent protein kinase activities during the acute exposure of the cells to the drugs. Growing the cells in the presence of opiates for several days led to drug addiction. In the tolerant-addicted cells, polyamine and cyclic AMP levels were close to normal values as were the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. Removal of the opiate from 'addicted' cells, by either washing or by adding the antagonist naloxone, resulted in an increase in cyclic AMP and polyamine levels and the activities of ornithine decarboxylase and cyclic AMP-dependent protein kinase. The effect of opiates was closely related to their biological activities. Inactive enantiomorphs did not affect cyclic AMP or polyamine levels; neither did they decrease ornithine decarboxylase and cyclic AMP-dependent protein kinase activities.

Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalBiochemical Journal
Volume227
Issue number2
DOIs
StatePublished - 1985

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