Optetrode: A multichannel readout for optogenetic control in freely moving mice

Polina Anikeeva, Aaron S. Andalman, Ilana Witten, Melissa Warden, Inbal Goshen, Logan Grosenick, Lisa A. Gunaydin, Loren M. Frank, Karl Deisseroth*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

292 Scopus citations


Recent advances in optogenetics have improved the precision with which defined circuit elements can be controlled optically in freely moving mammals; in particular, recombinase-dependent opsin viruses, used with a growing pool of transgenic mice expressing recombinases, allow manipulation of specific cell types. However, although optogenetic control has allowed neural circuits to be manipulated in increasingly powerful ways, combining optogenetic stimulation with simultaneous multichannel electrophysiological readout of isolated units in freely moving mice remains a challenge. We designed and validated the optetrode, a device that allows for colocalized multi-tetrode electrophysiological recording and optical stimulation in freely moving mice. Optetrode manufacture employs a unique optical fiber-centric coaxial design approach that yields a lightweight (2 g), compact and robust device that is suitable for behaving mice. This low-cost device is easy to construct (2.5 h to build without specialized equipment). We found that the drive design produced stable high-quality recordings and continued to do so for at least 6 weeks following implantation. We validated the optetrode by quantifying, for the first time, the response of cells in the medial prefrontal cortex to local optical excitation and inhibition, probing multiple different genetically defined classes of cells in the mouse during open field exploration.

Original languageAmerican English
Pages (from-to)163-170
Number of pages8
JournalNature Neuroscience
Issue number1
StatePublished - Jan 2012
Externally publishedYes

Bibliographical note

Funding Information:
We thank S. Arber for the PV::Cre transgenic mouse line. P.A. thanks D.G. Walker for advice on mechanical design. P.A. was supported by a Dean’s fellowship from Stanford University School of Medicine, I.W. was supported by the Helen Hay Whitney Foundation, I.G. was supported by a Machiah fellowship and the Weizmann Institute Women in Science award, L.G. was supported by a National Science Foundation Integrative Graduate Education and Research Traineeship Award, and L.A.G. was supported by a BioX fellowship from Stanford University. L.M.F. and K.D. received support from a GO grant from the National Institute of Neurological Disorders and Stroke. Full funding information for K.D. is listed at http://www.stanford.edu/group/dlab/optogenetics/funding/ and includes the Gatsby Charitable Foundation, the Defense Advanced Research Projects Agency Reorganization and Plasticity to Accelerate Injury Recovery Program, the California Institute for Regenerative Medicine, the McKnight Foundation, the National Institute of Mental Health, and the National Institute on Drug Abuse.


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