Optical and electrochemical detection of NADH and of NAD +-dependent biocatalyzed processes by the catalytic deposition of copper on gold nanoparticles

Bella Shlyahovsky*, Eugenii Katz, Yi Xiao, Valeri Pavlov, Itamar Willner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

The optical or electrical detection of 1,4-dihydro-β-nicotinamide adenine dinucleotide (NADH) and the sensing of ethanol by the NAD +-dependent alcohol dehydrogenase was analyzed. Metal nanoparticles (NPs) were employed as versatile labels for the optical or electrical detection of biorecognition events or biocatalytic transformations. Gold nanoparticles were prepared using NaBH 4 and stabilized with trisodium citrate. Ultrapure water from a NANOpure Diamond Barnstead source was also used. The conventional three-electrode cell consisting of the modified indium-tin oxide (ITO) working electrode, a glassy carbon auxiliary electrode isolated by a glass frit, and a saturated calomel reference electrode (SCE) connected to the working volume with a Luggin capillary, was used for the electrochemical measurements. Results show the catalytic deposition of Cu 0 on Au NPs by the NADH cofactor.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalSmall
Volume1
Issue number2
DOIs
StatePublished - Feb 2005

Keywords

  • Copper
  • Enzymes
  • Gold
  • Nanoparticles
  • Sensors

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