Optical assessment of nociceptive TRP channel function at the peripheral nerve terminal

Fernando Aleixandre-Carrera; Nurit Engelmayer; David Ares-Suárez; María del Carmen Acosta; Carlos Belmonte; Juana Gallar; Víctor Meseguer; Alexander M. Binshtok

doi:10.3390/ijms22020481

Optical assessment of nociceptive TRP channel function at the peripheral nerve terminal

Fernando Aleixandre-Carrera, Nurit Engelmayer, David Ares-Suárez, María del Carmen Acosta, Carlos Belmonte, Juana Gallar, Víctor Meseguer^*, Alexander M. Binshtok

^*Corresponding author for this work

Department of Medical Neurobiology

Research output: Contribution to journal › Review article › peer-review

3 Scopus citations

Abstract

Free nerve endings are key structures in sensory transduction of noxious stimuli. In spite of this, little is known about their functional organization. Transient receptor potential (TRP) channels have emerged as key molecular identities in the sensory transduction of pain-producing stimuli, yet the vast majority of our knowledge about sensory TRP channel function is limited to data obtained from in vitro models which do not necessarily reflect physiological conditions. In recent years, the development of novel optical methods such as genetically encoded calcium indicators and photo-modulation of ion channel activity by pharmacological tools has provided an invaluable opportunity to directly assess nociceptive TRP channel function at the nerve terminal.

Original language	American English
Article number	481
Pages (from-to)	1-20
Number of pages	20
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	2
DOIs	https://doi.org/10.3390/ijms22020481
State	Published - 2 Jan 2021

Bibliographical note

Funding Information:
Funding: This work was funded by The Spanish Agencia Estatal de Investigación and the European Regional Development Fund grants RTI2018-100994-AI00 and SAF2017-83674-C2-2-R and -1-R, The Spanish Ministerio de Universidades Fellowship FPU17/00483, The Generalitat Valenciana Excellence Project Program grant PROMETEO/2018/114, The Israeli Science Foundation, grant agreement 1470/17, Canadian Institutes of Health Research (CIHR), the International Development Research Centre (IDRC), the Israel Science Foundation (ISF) and the Azrieli Foundation, grant agreement 2545/18, and the Deutsch-Israelische Projectkooperation Program of the Deutsche Forschungsge-meinschaft (DIP), grant agreement BI 1665/1-1ZI1172/12-1.

Funding Information:
This work was funded by The Spanish Agencia Estatal de Investigaci?n and the European Regional Development Fund grants RTI2018-100994-AI00 and SAF2017-83674-C2-2-R and-1-R, The Spanish Ministerio de Universidades Fellowship FPU17/00483, The Generalitat Valenciana Excel-lence Project Program grant PROMETEO/2018/114, The Israeli Science Foundation, grant agreement 1470/17, Canadian Institutes of Health Research (CIHR), the International Development Research Centre (IDRC), the Israel Science Foundation (ISF) and the Azrieli Foundation, grant agreement 2545/18, and the Deutsch-Israelische Projectkooperation Program of the Deutsche Forschungsge-meinschaft (DIP), grant agreement BI 1665/1-1ZI1172/12-1.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Nociception
Optical recording
Opto-pharmacology
Pain
Peripheral nerve terminals
TRP channels
peripheral nerve terminals
optical recording
pain
nociception
opto-pharmacology

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title = "Optical assessment of nociceptive TRP channel function at the peripheral nerve terminal",

abstract = "Free nerve endings are key structures in sensory transduction of noxious stimuli. In spite of this, little is known about their functional organization. Transient receptor potential (TRP) channels have emerged as key molecular identities in the sensory transduction of pain-producing stimuli, yet the vast majority of our knowledge about sensory TRP channel function is limited to data obtained from in vitro models which do not necessarily reflect physiological conditions. In recent years, the development of novel optical methods such as genetically encoded calcium indicators and photo-modulation of ion channel activity by pharmacological tools has provided an invaluable opportunity to directly assess nociceptive TRP channel function at the nerve terminal.",

keywords = "Nociception, Optical recording, Opto-pharmacology, Pain, Peripheral nerve terminals, TRP channels, peripheral nerve terminals, optical recording, pain, nociception, opto-pharmacology",

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note = "Funding Information: Funding: This work was funded by The Spanish Agencia Estatal de Investigaci{\'o}n and the European Regional Development Fund grants RTI2018-100994-AI00 and SAF2017-83674-C2-2-R and -1-R, The Spanish Ministerio de Universidades Fellowship FPU17/00483, The Generalitat Valenciana Excellence Project Program grant PROMETEO/2018/114, The Israeli Science Foundation, grant agreement 1470/17, Canadian Institutes of Health Research (CIHR), the International Development Research Centre (IDRC), the Israel Science Foundation (ISF) and the Azrieli Foundation, grant agreement 2545/18, and the Deutsch-Israelische Projectkooperation Program of the Deutsche Forschungsge-meinschaft (DIP), grant agreement BI 1665/1-1ZI1172/12-1. Funding Information: This work was funded by The Spanish Agencia Estatal de Investigaci?n and the European Regional Development Fund grants RTI2018-100994-AI00 and SAF2017-83674-C2-2-R and-1-R, The Spanish Ministerio de Universidades Fellowship FPU17/00483, The Generalitat Valenciana Excel-lence Project Program grant PROMETEO/2018/114, The Israeli Science Foundation, grant agreement 1470/17, Canadian Institutes of Health Research (CIHR), the International Development Research Centre (IDRC), the Israel Science Foundation (ISF) and the Azrieli Foundation, grant agreement 2545/18, and the Deutsch-Israelische Projectkooperation Program of the Deutsche Forschungsge-meinschaft (DIP), grant agreement BI 1665/1-1ZI1172/12-1. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Acosta, María del Carmen

AU - Belmonte, Carlos

AU - Gallar, Juana

AU - Meseguer, Víctor

AU - Binshtok, Alexander M.

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N2 - Free nerve endings are key structures in sensory transduction of noxious stimuli. In spite of this, little is known about their functional organization. Transient receptor potential (TRP) channels have emerged as key molecular identities in the sensory transduction of pain-producing stimuli, yet the vast majority of our knowledge about sensory TRP channel function is limited to data obtained from in vitro models which do not necessarily reflect physiological conditions. In recent years, the development of novel optical methods such as genetically encoded calcium indicators and photo-modulation of ion channel activity by pharmacological tools has provided an invaluable opportunity to directly assess nociceptive TRP channel function at the nerve terminal.

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KW - Opto-pharmacology

KW - Pain

KW - Peripheral nerve terminals

KW - TRP channels

KW - peripheral nerve terminals

KW - optical recording

KW - pain

KW - nociception

KW - opto-pharmacology

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SP - 1

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 2

M1 - 481

ER -

Optical assessment of nociceptive TRP channel function at the peripheral nerve terminal

Abstract

Bibliographical note

Keywords

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