Optical biosensing of bacteria and cells using porous silicon based, photonic lamellar gratings

Y. Mirsky, A. Nahor, E. Edrei, N. Massad-Ivanir, L. M. Bonanno, E. Segal, A. Sa'Ar

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27 Scopus citations

Abstract

We report on a method to extend the optical sensing capabilities of conventional RIFTS (reflective interferometric Fourier transform spectroscopy) biosensors for real-time detection of large microorganisms, such as bacteria and cells. Using macro porous silicon based 2D arrays of phase (lamellar) grating, we demonstrate that the zero-order optical reflectivity exhibits a similar interference pattern to that obtained for ordinary RIFTS biosensors, which can be Fourier transformed into optical thickness and exploited for biosensing. The sensing capabilities are demonstrated for Escherichia coli bacteria that were captured inside the macro-pores. The entrapment process is monitored and verified by confocal laser scanning microscopy.

Original languageAmerican English
Article number033702
JournalApplied Physics Letters
Volume103
Issue number3
DOIs
StatePublished - 15 Jul 2013

Bibliographical note

Funding Information:
This work has been partially supported by grants from the Israeli ministry of science (Grant No. 3-6798), and from the Israel Science Foundation (ISF), Grant No. 425/09. A.N. acknowledges the CAMBR foundation for the support. E.S., N.M.I., and L.M.B. are grateful to the support of the Larry I. Lokey Interdisciplinary Center for Life Sciences and Engineering and the Russell Berrie Nanotechnology Institute.

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