TY - JOUR
T1 - Optical modeling of bioluminescence in whole cell biosensors
AU - Ben-Yoav, Hadar
AU - Elad, Tal
AU - Shlomovits, Omer
AU - Belkin, Shimshon
AU - Shacham-Diamand, Yosi
PY - 2009/3/15
Y1 - 2009/3/15
N2 - Bioluminescence-based whole cell biosensors are devices that can be very useful for environmental monitoring applications. The advantages of these devices are that they can be produced as a single-chip, low-power, rugged, inexpensive component, and can be deployed in a variety of non-laboratory settings. However, such biosensors encounter inherent problems in overall system light collection efficiency. The light emitted from the bioluminescent microbial cells is isotropic and passes through various media before it reaches the photon detectors. We studied the bioluminescence distribution and propagation in microbial whole cell biochips. Optical emission and detection were modeled and simulated using an optical ray tracing method. Light emission, transfer and detection were simulated and optimized with respect to two fundamental system parameters: system geometry and bacterial concentration. Optimization elucidated some of the optical aspects of the biochip, e.g. detector radius values between 300 and 750 μm, and bacterial fixation radius values between 800 and 1200 μm. Understanding theses aspects may establish a basis for future optimization of similar chips.
AB - Bioluminescence-based whole cell biosensors are devices that can be very useful for environmental monitoring applications. The advantages of these devices are that they can be produced as a single-chip, low-power, rugged, inexpensive component, and can be deployed in a variety of non-laboratory settings. However, such biosensors encounter inherent problems in overall system light collection efficiency. The light emitted from the bioluminescent microbial cells is isotropic and passes through various media before it reaches the photon detectors. We studied the bioluminescence distribution and propagation in microbial whole cell biochips. Optical emission and detection were modeled and simulated using an optical ray tracing method. Light emission, transfer and detection were simulated and optimized with respect to two fundamental system parameters: system geometry and bacterial concentration. Optimization elucidated some of the optical aspects of the biochip, e.g. detector radius values between 300 and 750 μm, and bacterial fixation radius values between 800 and 1200 μm. Understanding theses aspects may establish a basis for future optimization of similar chips.
KW - Biochip
KW - Bioluminescence
KW - Optical modeling
KW - Stray light analysis
KW - Water toxicity
KW - Whole cell biosensor
UR - http://www.scopus.com/inward/record.url?scp=60349087846&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2008.10.035
DO - 10.1016/j.bios.2008.10.035
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C2 - 19131239
AN - SCOPUS:60349087846
SN - 0956-5663
VL - 24
SP - 1969
EP - 1973
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 7
ER -