Optofluidic Sensor for Inline Hemolysis Detection on Whole Blood

Chen Zhou, Mehdi Keshavarz Hedayati, Xiaolong Zhu, Frank Nielsen, Uriel Levy, Anders Kristensen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Hemolysis is the rupture of red blood cells and constitutes the most common reason for unsuitable blood samples in the clinic. To detect hemolysis, one has to separate the hemoglobin in blood plasma from that in red blood cells. However, current methods entail centrifugation for cell-plasma separation, which is complex, time-consuming, and not easy to integrate into point-of-care (PoC) systems. Here, we demonstrate an optofluidic sensor composed of nanofilters on an optical waveguide, which enables evanescent-wave absorption measurement of hemoglobin in plasma with the capability of real-time inline detection on whole blood without extra sample preparation like centrifugation. Long-term testing with inline integration in a modified, commercial blood gas analyzer shows high reliability and repeatability of the measurements even with the presence of interference from bilirubin. We envision that the present work has large potential in improving diagnosis quality by enabling PoC hemolysis detection in blood gas analyzers and can also lend unique sensing capabilities to other applications dealing with complex turbid media.

Original languageAmerican English
Pages (from-to)784-791
Number of pages8
JournalACS Sensors
Volume3
Issue number4
DOIs
StatePublished - 27 Apr 2018

Bibliographical note

Publisher Copyright:
© 2018 American Chemical Society.

Keywords

  • Point-of-Care
  • hemolysis sensor
  • in vitro diagnostics
  • nanoimprint
  • nanowells
  • optofluidics
  • waveguide sensor
  • whole blood

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