Optogenetic inhibition of cocaine seeking in rats

Michael T. Stefanik, Khaled Moussawi, Yonatan M. Kupchik, Kyle C. Smith, Rachel L. Miller, Mary L. Huff, Karl Deisseroth, Peter W. Kalivas*, Ryan T. Lalumiere

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

190 Scopus citations

Abstract

Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self-administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking.

Original languageAmerican English
Pages (from-to)50-53
Number of pages4
JournalAddiction Biology
Volume18
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

Keywords

  • ArchT
  • eNpHR3.0
  • halorhodopsin
  • nucleus accumbens core
  • prelimbic cortex
  • reinstatement.

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