Optogenetic inhibition of cocaine seeking in rats

Michael T. Stefanik; Khaled Moussawi; Yonatan M. Kupchik; Kyle C. Smith; Rachel L. Miller; Mary L. Huff; Karl Deisseroth; Peter W. Kalivas; Ryan T. Lalumiere

doi:10.1111/j.1369-1600.2012.00479.x

Optogenetic inhibition of cocaine seeking in rats

Michael T. Stefanik, Khaled Moussawi, Yonatan M. Kupchik, Kyle C. Smith, Rachel L. Miller, Mary L. Huff, Karl Deisseroth, Peter W. Kalivas^*, Ryan T. Lalumiere

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

198 Scopus citations

Abstract

Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self-administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking.

Original language	English
Pages (from-to)	50-53
Number of pages	4
Journal	Addiction Biology
Volume	18
Issue number	1
DOIs	https://doi.org/10.1111/j.1369-1600.2012.00479.x
State	Published - Jan 2013
Externally published	Yes

Keywords

ArchT
eNpHR3.0
halorhodopsin
nucleus accumbens core
prelimbic cortex
reinstatement.

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10.1111/j.1369-1600.2012.00479.x

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title = "Optogenetic inhibition of cocaine seeking in rats",

abstract = "Inhibitory optogenetics was used to examine the roles of the prelimbic cortex (PL), the nucleus accumbens core (NAcore) and the PL projections to the NAcore in the reinstatement of cocaine seeking. Rats were microinjected into the PL or NAcore with an adeno-associated virus containing halorhodopsin or archaerhodopsin. After 12 days of cocaine self-administration, followed by extinction training, animals underwent reinstatement testing along with the presence/absence of optically induced inhibition via laser light. Bilateral optical inhibition of the PL, NAcore or the PL fibers in the NAcore inhibited the reinstatement of cocaine seeking.",

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AU - Deisseroth, Karl

AU - Kalivas, Peter W.

AU - Lalumiere, Ryan T.

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Optogenetic inhibition of cocaine seeking in rats

Abstract

Keywords

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