Organization of 5-Methylcytosine in Chromosomal DNA

Adinah Solage; Howard Cedar

doi:10.1021/bi00607a036

Organization of 5-Methylcytosine in Chromosomal DNA

Adinah Solage, Howard Cedar^*

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The 5-methylcytosine residues of L-cells have been labeled with [methyl-³H]-l-methionine and their chromatin localization studied using deoxyribonucleases. The kinetics of micrococcal nuclease digestion showed that the methylated cytosine residues are concentrated within regions resistant to nuclease digestion and preferentially missing from those regions between nucleosomes which are nuclease sensitive. Using DNA hybridization kinetic analysis, it is shown that 5-methylcytosine is abundant in highly repeated sequences but is also present in middle repetitive and unique sequence DNA.

Original language	English
Pages (from-to)	2934-2938
Number of pages	5
Journal	Biochemistry
Volume	17
Issue number	14
DOIs	https://doi.org/10.1021/bi00607a036
State	Published - 1978

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author = "Adinah Solage and Howard Cedar",

year = "1978",

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AB - The 5-methylcytosine residues of L-cells have been labeled with [methyl-3H]-l-methionine and their chromatin localization studied using deoxyribonucleases. The kinetics of micrococcal nuclease digestion showed that the methylated cytosine residues are concentrated within regions resistant to nuclease digestion and preferentially missing from those regions between nucleosomes which are nuclease sensitive. Using DNA hybridization kinetic analysis, it is shown that 5-methylcytosine is abundant in highly repeated sequences but is also present in middle repetitive and unique sequence DNA.

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Organization of 5-Methylcytosine in Chromosomal DNA

Abstract

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