TY - JOUR
T1 - Ornatilinea apprima gen. nov., sp. nov., a cellulolytic representative of the class Anaerolineae
AU - Podosokorskaya, O. A.
AU - Bonch-Osmolovskaya, E. A.
AU - Novikov, A. A.
AU - Kolganova, T. V.
AU - Kublanov, I. V.
PY - 2013
Y1 - 2013
N2 - A novel obligately anaerobic, mesophilic, organotrophic bacterium, strain P3M-1T, was isolated from a microbial mat formed in a wooden bath filled with hot water emerging from a 2775 mdeep well in the Tomsk region of western Siberia, Russia. Cells of strain P3M-1T were rodshaped, 0.3-0.7 μm in width and formed multicellullar filaments that reached up to 400 mm in length. Strain P3M-1T grew optimally at 42-45 °C, pH 7.5-8.0, and with 0.1% (w/v) NaCl. Under optimal conditions, the doubling time was 6 h. The isolate was able to ferment a variety of proteinaceous substrates and sugars, including microcrystalline cellulose. Acetate, ethanol and H2 were the main products of glucose fermentation. The genomic DNA G+C content was 55 mol%. 16S rRNA gene sequence-based phylogenetic analyses showed that strain P3M-1T was a member of the class Anaerolinea, with 92.8% sequence similarity to Levilinea saccharolytica KIBI-1T. Based on phylogenetic analysis and physiological properties, strain P3M-1T represents a novel species in a new genus, for which the name Ornatilinea apprima gen. nov., sp. nov. is proposed; the type strain of O. apprima is P3M-1T (5DSM 23815T5VKM B-2669T).
AB - A novel obligately anaerobic, mesophilic, organotrophic bacterium, strain P3M-1T, was isolated from a microbial mat formed in a wooden bath filled with hot water emerging from a 2775 mdeep well in the Tomsk region of western Siberia, Russia. Cells of strain P3M-1T were rodshaped, 0.3-0.7 μm in width and formed multicellullar filaments that reached up to 400 mm in length. Strain P3M-1T grew optimally at 42-45 °C, pH 7.5-8.0, and with 0.1% (w/v) NaCl. Under optimal conditions, the doubling time was 6 h. The isolate was able to ferment a variety of proteinaceous substrates and sugars, including microcrystalline cellulose. Acetate, ethanol and H2 were the main products of glucose fermentation. The genomic DNA G+C content was 55 mol%. 16S rRNA gene sequence-based phylogenetic analyses showed that strain P3M-1T was a member of the class Anaerolinea, with 92.8% sequence similarity to Levilinea saccharolytica KIBI-1T. Based on phylogenetic analysis and physiological properties, strain P3M-1T represents a novel species in a new genus, for which the name Ornatilinea apprima gen. nov., sp. nov. is proposed; the type strain of O. apprima is P3M-1T (5DSM 23815T5VKM B-2669T).
UR - http://www.scopus.com/inward/record.url?scp=84872484014&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.041012-0
DO - 10.1099/ijs.0.041012-0
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C2 - 22328612
AN - SCOPUS:84872484014
SN - 1466-5026
VL - 63
SP - 86
EP - 92
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
ER -