Osmotic stress mechanically perturbs chemoreceptors in Escherichia coli

Ady Vaknin, Howard C. Berg

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Two-component signaling systems play a major role in the long-term adaptation of microorganisms to changes in osmolarity, but how osmoreceptors work is not well understood. Temporal changes in solute concentration are sensed by the chemotaxis system in Escherichia coli, enabling these bacteria to avoid regions of high osmolarity. To study how osmolarity is detected in this system, we fused yellow fluorescent protein (YFP) to the C terminus of the serine or aspartate chemoreceptor, monitored the steady-state fluorescence polarization of YFP, and found that the polarization decreased substantially upon addition of osmotic agents. This decrease was due to an increase in fluorescence resonance energy transfer between YFP fluorophores in adjacent homodimers within trimers of dimers. Thus, changes in homodimer spacing and/or orientation appear to initiate osmotactic signaling.

Original languageAmerican English
Pages (from-to)592-596
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number3
DOIs
StatePublished - 17 Jan 2006

Keywords

  • Bacteria
  • Fluorescence depolarization
  • Osmolarity
  • Signal transduction
  • Yellow fluorescent protein

Fingerprint

Dive into the research topics of 'Osmotic stress mechanically perturbs chemoreceptors in Escherichia coli'. Together they form a unique fingerprint.

Cite this