TY - JOUR
T1 - Ovarian substance induces steroid production in cultured granulosa cells
AU - Orly, Joseph
AU - Farkash, Yigal
AU - Hershkovits, Nitzan
AU - Mizrahi, Lina
AU - Weinberger, Patricia
PY - 1982/12
Y1 - 1982/12
N2 - The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on, intensive progestin production. However, unlike FSH, OS does not induce accumulation of cyclic AMP (cAMP) in the granulosa cells. The ovarian factor cannot be cAMP as its action is not abolished by phosphodiesterase (PDE) treatment. Neither is it a possible PDE inhibitor, as it does not augment cAMP accumulation in granulosa cells or Friend erythroleukemic cells induced by FSH or PGE1, respectively. The factor is still active after heating for 20 min at 90° C but is rapidly inactivated by alkali treatment. In addition, treatment with various proteases did not abolish the steroidogenic activity. These findings suggest a possible novel intraovarian regulator of the granulosa cell function.
AB - The rat ovary produces an apparently low molecular weight substance that mimics the action of follitropin (FSH) on ovarian granulosa cells in culture. Similar to FSH action, the ovarian substance (OS) induces temporal cell rounding and, later on, intensive progestin production. However, unlike FSH, OS does not induce accumulation of cyclic AMP (cAMP) in the granulosa cells. The ovarian factor cannot be cAMP as its action is not abolished by phosphodiesterase (PDE) treatment. Neither is it a possible PDE inhibitor, as it does not augment cAMP accumulation in granulosa cells or Friend erythroleukemic cells induced by FSH or PGE1, respectively. The factor is still active after heating for 20 min at 90° C but is rapidly inactivated by alkali treatment. In addition, treatment with various proteases did not abolish the steroidogenic activity. These findings suggest a possible novel intraovarian regulator of the granulosa cell function.
KW - cell culture
KW - cyclic AMP
KW - granulosa
KW - phosphodiesterase
KW - steroidogenesis
UR - http://www.scopus.com/inward/record.url?scp=0020307586&partnerID=8YFLogxK
U2 - 10.1007/BF02796372
DO - 10.1007/BF02796372
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C2 - 6299929
AN - SCOPUS:0020307586
SN - 0073-5655
VL - 18
SP - 980
EP - 989
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
IS - 12
ER -