Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus

Linda K. Gont; Abraham Fainsod; Sung Hyun Kim; Eddy M. De Robertis

doi:10.1006/dbio.1996.0061

Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus

Linda K. Gont, Abraham Fainsod^*, Sung Hyun Kim, Eddy M. De Robertis

^*Corresponding author for this work

Department of Developmental Biology and Cancer Research

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Xnot-2 is a homeobox gene expressed in Spemann's organizer. here we present evidence that microinjection of synthetic Xnot-2 mRNA leads to the formation of notochord. Microinjection into the dorsal side of the Xenopus embryo results in greatly expanded notochords. Nearby somitic and prechordal mesoderm becomes recruited into these enlarged notochords, which also affect CNS patterning. Two early genes expressed in the developing notochord, chd and XFKH-1, are activated by Xnot-2. We conclude that gain-of-function of Xnot-2 promotes notochord formation.

Original language	English
Pages (from-to)	174-178
Number of pages	5
Journal	Developmental Biology
Volume	174
Issue number	1
DOIs	https://doi.org/10.1006/dbio.1996.0061
State	Published - 1996

Bibliographical note

Funding Information:
We thank Bin Lu, Yoshiki Sasai, and Tewis Bouwmeester for advice and Anatalia Cuellar for technical assistance. This work was supported by the NIH (HD21502-10). E.D.R. is an Investigator of the Howard Hughes Medical Institute.

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abstract = "Xnot-2 is a homeobox gene expressed in Spemann's organizer. here we present evidence that microinjection of synthetic Xnot-2 mRNA leads to the formation of notochord. Microinjection into the dorsal side of the Xenopus embryo results in greatly expanded notochords. Nearby somitic and prechordal mesoderm becomes recruited into these enlarged notochords, which also affect CNS patterning. Two early genes expressed in the developing notochord, chd and XFKH-1, are activated by Xnot-2. We conclude that gain-of-function of Xnot-2 promotes notochord formation.",

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AU - De Robertis, Eddy M.

N1 - Funding Information: We thank Bin Lu, Yoshiki Sasai, and Tewis Bouwmeester for advice and Anatalia Cuellar for technical assistance. This work was supported by the NIH (HD21502-10). E.D.R. is an Investigator of the Howard Hughes Medical Institute.

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AB - Xnot-2 is a homeobox gene expressed in Spemann's organizer. here we present evidence that microinjection of synthetic Xnot-2 mRNA leads to the formation of notochord. Microinjection into the dorsal side of the Xenopus embryo results in greatly expanded notochords. Nearby somitic and prechordal mesoderm becomes recruited into these enlarged notochords, which also affect CNS patterning. Two early genes expressed in the developing notochord, chd and XFKH-1, are activated by Xnot-2. We conclude that gain-of-function of Xnot-2 promotes notochord formation.

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Overexpression of the homeobox gene Xnot-2 leads to notochord formation in Xenopus

Abstract

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