Packaging of DNA origami in viral capsids

Idit Kopatz; Ran Zalk; Yael Levi-Kalisman; Efrat Zlotkin-Rivkin; Gabriel A. Frank; Stanislav Kler

doi:10.1039/c8nr10113b

Packaging of DNA origami in viral capsids

Idit Kopatz^*
, Ran Zalk
, Yael Levi-Kalisman
, Efrat Zlotkin-Rivkin
, Gabriel A. Frank
, Stanislav Kler

^*Corresponding author for this work

Center for Nanoscience and Nanotechnology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

Original language	English
Pages (from-to)	10160-10166
Number of pages	7
Journal	Nanoscale
Volume	11
Issue number	21
DOIs	https://doi.org/10.1039/c8nr10113b
State	Published - 7 Jun 2019

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© 2019 The Royal Society of Chemistry.

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abstract = "Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.",

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AU - Levi-Kalisman, Yael

AU - Zlotkin-Rivkin, Efrat

AU - Frank, Gabriel A.

AU - Kler, Stanislav

PY - 2019/6/7

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AB - Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

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Packaging of DNA origami in viral capsids

Abstract

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