TY - JOUR
T1 - Paclitaxel palmitate-loaded anti-HER2 cationic immunoemulsion
T2 - Pharmacokinetic and biodistribution study in healthy mice
AU - Goldstein, D.
AU - Benita, S.
PY - 2009
Y1 - 2009
N2 - The aim of this study was to evaluate the pharmacokinetic and biodistribution profile of paclitaxel palmitate (PCPL)-loadedanti-HER2 cationic immunoemulsion in healthy mice as compared to PCPL solution and PCPL-loaded cationic emulsion following intravenous (TV) injection. The observed density of the monoclonal antibody (MAb) conjugated to the cationic emulsion was found to be around 35 MAbs/droplet (70% coupling efficiency). Both PCPL-loaded cationic emulsion and immunoemulsion showed significant increases in tl/2, Cmax and AUC values as compared to the values of PCPL solution in mice. There was no significant difference in the t1/2 and C max values between PCPL-loaded cationic emulsion and PCPL-loaded immunoemulsion. However, the PCPL-loaded immunoemulsion concentrated much less in the liver and spleen than PCPL-loaded cationic emulsion. The pharmacokinetic behavior of the immunoemulsion was markedly different from the pharmacokinetic profile of the naked MAb showing that the MAb lost its intrinsic molecular pharmacokinetic properties following conjugation to the cationic emulsion.
AB - The aim of this study was to evaluate the pharmacokinetic and biodistribution profile of paclitaxel palmitate (PCPL)-loadedanti-HER2 cationic immunoemulsion in healthy mice as compared to PCPL solution and PCPL-loaded cationic emulsion following intravenous (TV) injection. The observed density of the monoclonal antibody (MAb) conjugated to the cationic emulsion was found to be around 35 MAbs/droplet (70% coupling efficiency). Both PCPL-loaded cationic emulsion and immunoemulsion showed significant increases in tl/2, Cmax and AUC values as compared to the values of PCPL solution in mice. There was no significant difference in the t1/2 and C max values between PCPL-loaded cationic emulsion and PCPL-loaded immunoemulsion. However, the PCPL-loaded immunoemulsion concentrated much less in the liver and spleen than PCPL-loaded cationic emulsion. The pharmacokinetic behavior of the immunoemulsion was markedly different from the pharmacokinetic profile of the naked MAb showing that the MAb lost its intrinsic molecular pharmacokinetic properties following conjugation to the cationic emulsion.
KW - Cationic emulsion
KW - Immunoconjugates
KW - Monoclonal antibodies
KW - Paclitaxel palmitate
KW - Pharmacokinetics
UR - http://www.scopus.com/inward/record.url?scp=69249138356&partnerID=8YFLogxK
U2 - 10.1016/S1773-2247(09)50051-2
DO - 10.1016/S1773-2247(09)50051-2
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AN - SCOPUS:69249138356
SN - 1773-2247
VL - 19
SP - 269
EP - 274
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
IS - 4
ER -