TY - JOUR
T1 - Paraquat toxicity in Pisum sativum
T2 - Effects on soluble and membrane‐bound proteins
AU - Peleg, Itzhak
AU - Zer, Hagit
AU - Chevion, Mordechal
PY - 1992/9
Y1 - 1992/9
N2 - The effects of paraquat (PQ) on Pisum sativum L. proteins were investigated in vivo in a new experimental system utilizing 10‐day‐old plant cuts.A marked decrease in the specific activity of membrane‐bound Ca2+‐dependent ATPase was recorded, while that of Mg2+‐dependent ATPase remained unchanged. Concurrently with a drop in the total plant protein, the specific activities of the three cytoplasmic enzymes, malate dehydrogenase, hydroxypyruvate reductase and triose‐phosphate isomerase, were also found to decrease. The effect on various enzymes involved in cellular defense mechanisms was also studied: glutathione reductase and superoxide dismutase activities increased, while ascorbate peroxidase was not affected. These findings shed light on the selectivity of PQ‐induced injurious processes, focusing on protein homeostasis mechanisms in the membrane and cytoplasmic compartments at the cellular level, as well as on the prominent role played by enzymatic defense systems against PQ poisoning.
AB - The effects of paraquat (PQ) on Pisum sativum L. proteins were investigated in vivo in a new experimental system utilizing 10‐day‐old plant cuts.A marked decrease in the specific activity of membrane‐bound Ca2+‐dependent ATPase was recorded, while that of Mg2+‐dependent ATPase remained unchanged. Concurrently with a drop in the total plant protein, the specific activities of the three cytoplasmic enzymes, malate dehydrogenase, hydroxypyruvate reductase and triose‐phosphate isomerase, were also found to decrease. The effect on various enzymes involved in cellular defense mechanisms was also studied: glutathione reductase and superoxide dismutase activities increased, while ascorbate peroxidase was not affected. These findings shed light on the selectivity of PQ‐induced injurious processes, focusing on protein homeostasis mechanisms in the membrane and cytoplasmic compartments at the cellular level, as well as on the prominent role played by enzymatic defense systems against PQ poisoning.
KW - Ascorbate peroxidase
KW - ATPase
KW - glutathione reductase
KW - paraquat
KW - pea
KW - Pisum sativum
KW - protein damage
KW - protein profile
KW - superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=84989754264&partnerID=8YFLogxK
U2 - 10.1111/j.1399-3054.1992.tb01321.x
DO - 10.1111/j.1399-3054.1992.tb01321.x
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AN - SCOPUS:84989754264
SN - 0031-9317
VL - 86
SP - 131
EP - 135
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 1
ER -