Parasite antigens recognized by patients with cutaneous leishmaniasis

C. L. Jaffe*, R. Shor, H. Trau, J. H. Passwell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Humoral and cell-mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophosphoglycan, both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen-specific responses to a 72-82 kD component in promastigote lysates.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalClinical and Experimental Immunology
Volume80
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • Leishmania major
  • lipophosphoglycan
  • lymphocyte proliferation
  • parasite surface protease

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