TY - JOUR
T1 - Parasite antigens recognized by patients with cutaneous leishmaniasis
AU - Jaffe, C. L.
AU - Shor, R.
AU - Trau, H.
AU - Passwell, J. H.
PY - 1990
Y1 - 1990
N2 - Humoral and cell-mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophosphoglycan, both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen-specific responses to a 72-82 kD component in promastigote lysates.
AB - Humoral and cell-mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10000 and recognized multiple components by Western blotting with molecular weights between 5000 and >200000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophosphoglycan, both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 μg/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen-specific responses to a 72-82 kD component in promastigote lysates.
KW - Leishmania major
KW - lipophosphoglycan
KW - lymphocyte proliferation
KW - parasite surface protease
UR - http://www.scopus.com/inward/record.url?scp=0025344752&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2249.1990.tb06444.x
DO - 10.1111/j.1365-2249.1990.tb06444.x
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2323102
AN - SCOPUS:0025344752
SN - 0009-9104
VL - 80
SP - 77
EP - 82
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 1
ER -