TY - JOUR
T1 - Patterns of mobilization of copper and iron following myocardial ischemia
T2 - Possible predictive criteria for tissue injury
AU - Berenshtein, Eduard
AU - Mayer, Bernd
AU - Goldberg, Chaya
AU - Kitrossky, Nahum
AU - Chevion, Mordechai
PY - 1997/11
Y1 - 1997/11
N2 - Direct evidence for substantial iron and copper mobilization into the coronary now immediately following prolonged, but not short, cardiac ischemia is presented. When small volumes of coronary flow fractions (CFFs) were serially collected upon reperfusion, after 25-60 min of ischemia the copper and iron levels in the first CFF were 50-fold and 12- to 15-fold higher, respectively, than corresponding pre-ischemic values. The copper and iron levels after shorter periods (15-21 min) of ischemia were only about fivefold higher than the pre-ischemic values. This demonstrates that the resumption of coronary flow is accompanied by a burst of both metal ions. The levels of Cu/Fe in the CFFs correlated well with the loss of cardiac function following global ischemia of varying duration. After 18 min of ischemia, the residual cardiac function was less than 50%, and the damage was essentially reversible. After 25 min of ischemia, it exceeded 50% and was only partially reversible, while after 35 min, the damage exceeded 80%, and was mostly irreversible. The results are in accord with the hypothesis that copper and iron play causative roles in myocardial injury through mediation of hydroxyl radical production. Thus, the pattern of Cu/Fe mobilization from the tissue into the CFF can be used for the prediction of the severity of myocardial damage following ischemia, and could be developed into useful modalities for intervention in tissue injury.
AB - Direct evidence for substantial iron and copper mobilization into the coronary now immediately following prolonged, but not short, cardiac ischemia is presented. When small volumes of coronary flow fractions (CFFs) were serially collected upon reperfusion, after 25-60 min of ischemia the copper and iron levels in the first CFF were 50-fold and 12- to 15-fold higher, respectively, than corresponding pre-ischemic values. The copper and iron levels after shorter periods (15-21 min) of ischemia were only about fivefold higher than the pre-ischemic values. This demonstrates that the resumption of coronary flow is accompanied by a burst of both metal ions. The levels of Cu/Fe in the CFFs correlated well with the loss of cardiac function following global ischemia of varying duration. After 18 min of ischemia, the residual cardiac function was less than 50%, and the damage was essentially reversible. After 25 min of ischemia, it exceeded 50% and was only partially reversible, while after 35 min, the damage exceeded 80%, and was mostly irreversible. The results are in accord with the hypothesis that copper and iron play causative roles in myocardial injury through mediation of hydroxyl radical production. Thus, the pattern of Cu/Fe mobilization from the tissue into the CFF can be used for the prediction of the severity of myocardial damage following ischemia, and could be developed into useful modalities for intervention in tissue injury.
KW - Copper
KW - Free radicals
KW - Iron
KW - Ischemia
KW - Isolated heart
KW - Metal mobilization
KW - Reperfusion
UR - http://www.scopus.com/inward/record.url?scp=0031281739&partnerID=8YFLogxK
U2 - 10.1006/jmcc.1997.0535
DO - 10.1006/jmcc.1997.0535
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C2 - 9405177
AN - SCOPUS:0031281739
SN - 0022-2828
VL - 29
SP - 3025
EP - 3034
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 11
ER -