Pelagibacterium halotolerans gen. nov., sp. nov. and Pelagibacterium luteolum sp. nov., novel members of the family hyphomicrobiaceae

Xue Wei Xu*, Ying Yi Huo, Chun Sheng Wang, Aharon Oren, Heng Lin Cui, Eve Vedler, Min Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Two Gram-negative, motile, aerobic bacterial strains, designated B2 T and 1_C16_27 T, were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C 19: 0 cyclo), octadecanoic (C 18: 0 and C 18: 0 3-OH), octadecenoic (C 18: 1) and hexadecanoic (C 16: 0) acids. The G+C content of the genomic DNA was 58.1-59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family Hyphomicrobiaceae. The phylogenetically closest relatives were Cucumibacter (92.7-93.7% 16S rRNA gene sequence similarity), Devosia (92.9-94.4%) and Zhangella (91.7-92.1%). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2 T and 1_C16_27 T could be differentiated from each other and from members of the genera Cucumibacter, Devosia and Zhangella. Therefore, it is proposed that strains B2 T and 1_C16_27 T represent two novel species in a new genus, for which the names Pelagibacterium halotolerans gen. nov., sp. nov. (the type species; type strain B2 T 5CGMCC 1.7692 T 5JCM 15775 T) and Pelagibacterium luteolum sp. nov. (type strain 1_C16_27 T 5CGMCC 1.10267 T 5JCM 16552 T 5CELMS EEUT 1C1627 T) are proposed.

Original languageAmerican English
Pages (from-to)1817-1822
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number8
DOIs
StatePublished - Aug 2011

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