TY - JOUR
T1 - Peptidyl transfer RNA VI. The chemical synthesis of tri- and tetrapeptidyl transfer RNA
AU - Lapidot, Y.
AU - De Groot, N.
AU - Rappoport, S.
PY - 1969/5/20
Y1 - 1969/5/20
N2 - A general method for the preparation of tripeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-monomethoxytrityldipeptide and aminoacyl-tRNA. The monomethoxytrityl group is removed from the blocked peptidyl-tRNA under very mild conditions (5% dichloroacetic acid for 5 min at 4°) leaving the tRNA intact. When N-blocked carboxyl-activated tripeptide was reacted with aminoacyl-tRNA and the product formed was treated with dichloroacetic acid, a tetrapeptidyl-tRNA was obtained. The peptidyl-tRNA's described in this communication contained only bifunctional amino acids such as glycine, alanine, valine and phenylalanine. The activated N-blocked dipeptides used were either composed of two identical amino acids as in alanylalanine or of two different amino acids as in glycylphenylalanine.
AB - A general method for the preparation of tripeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-monomethoxytrityldipeptide and aminoacyl-tRNA. The monomethoxytrityl group is removed from the blocked peptidyl-tRNA under very mild conditions (5% dichloroacetic acid for 5 min at 4°) leaving the tRNA intact. When N-blocked carboxyl-activated tripeptide was reacted with aminoacyl-tRNA and the product formed was treated with dichloroacetic acid, a tetrapeptidyl-tRNA was obtained. The peptidyl-tRNA's described in this communication contained only bifunctional amino acids such as glycine, alanine, valine and phenylalanine. The activated N-blocked dipeptides used were either composed of two identical amino acids as in alanylalanine or of two different amino acids as in glycylphenylalanine.
UR - http://www.scopus.com/inward/record.url?scp=0014685117&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(69)90525-5
DO - 10.1016/0005-2787(69)90525-5
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C2 - 5797371
AN - SCOPUS:0014685117
SN - 0005-2787
VL - 182
SP - 105
EP - 112
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 1
ER -