Peptidyl transfer RNA VIII. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA

Y. Lapidot; N. De Groot; S. Rappoport; D. Elat

doi:10.1016/0005-2787(69)90081-1

Peptidyl transfer RNA VIII. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA

Y. Lapidot^*, N. De Groot, S. Rappoport, D. Elat

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

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Abstract

The preparation of alanine and glycine oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of mono-p-methoxytrityl-Gly_n (n = 1-3) or mono-p-methoxytrityl-Ala_n (n = 1-5) and aminoacyl-tRNA and subsequent removal of the N-protecting group by mild acidic treatment (5% dichloroacetic acid at 4°). The acylation reaction was repeated up to 4 times resulting in the formation of oligopeptidyl-tRNA of the type Gly_n aminoacyl-tRNA (n = 1-12) and Ala_n aminoacyl-tRNA (n = 1-9).

Original language	English
Pages (from-to)	304-311
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(69)90081-1
State	Published - 22 Oct 1969

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abstract = "The preparation of alanine and glycine oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of mono-p-methoxytrityl-Glyn (n = 1-3) or mono-p-methoxytrityl-Alan (n = 1-5) and aminoacyl-tRNA and subsequent removal of the N-protecting group by mild acidic treatment (5% dichloroacetic acid at 4°). The acylation reaction was repeated up to 4 times resulting in the formation of oligopeptidyl-tRNA of the type Glyn aminoacyl-tRNA (n = 1-12) and Alan aminoacyl-tRNA (n = 1-9).",

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T1 - Peptidyl transfer RNA VIII. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA

AU - Lapidot, Y.

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AU - Elat, D.

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N2 - The preparation of alanine and glycine oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of mono-p-methoxytrityl-Glyn (n = 1-3) or mono-p-methoxytrityl-Alan (n = 1-5) and aminoacyl-tRNA and subsequent removal of the N-protecting group by mild acidic treatment (5% dichloroacetic acid at 4°). The acylation reaction was repeated up to 4 times resulting in the formation of oligopeptidyl-tRNA of the type Glyn aminoacyl-tRNA (n = 1-12) and Alan aminoacyl-tRNA (n = 1-9).

AB - The preparation of alanine and glycine oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of mono-p-methoxytrityl-Glyn (n = 1-3) or mono-p-methoxytrityl-Alan (n = 1-5) and aminoacyl-tRNA and subsequent removal of the N-protecting group by mild acidic treatment (5% dichloroacetic acid at 4°). The acylation reaction was repeated up to 4 times resulting in the formation of oligopeptidyl-tRNA of the type Glyn aminoacyl-tRNA (n = 1-12) and Alan aminoacyl-tRNA (n = 1-9).

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Peptidyl transfer RNA VIII. The chemical synthesis of glycine and alanine containing oligopeptidyl transfer RNA

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