Peptidyl transfer RNA. X. The chemical synthesis of peptidyl transfer RNA containing hydroxy amino acids

M. Rubinstein*, N. de Groot, Y. Lapidot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serinen (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)6-Phe-tRNA.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume209
Issue number1
DOIs
StatePublished - 21 May 1970

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