Abstract
The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serinen (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)6-Phe-tRNA.
Original language | English |
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Pages (from-to) | 183-189 |
Number of pages | 7 |
Journal | BBA Section Nucleic Acids And Protein Synthesis |
Volume | 209 |
Issue number | 1 |
DOIs | |
State | Published - 21 May 1970 |