Peptidyl transfer RNA. X. The chemical synthesis of peptidyl transfer RNA containing hydroxy amino acids

M. Rubinstein; N. de Groot; Y. Lapidot

doi:10.1016/0005-2787(70)90674-X

Peptidyl transfer RNA. X. The chemical synthesis of peptidyl transfer RNA containing hydroxy amino acids

M. Rubinstein^*
, N. de Groot
, Y. Lapidot

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serine_n (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)_n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na₂S₂O₃. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)₆-Phe-tRNA.

Original language	English
Pages (from-to)	183-189
Number of pages	7
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	209
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(70)90674-X
State	Published - 21 May 1970

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abstract = "The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serinen (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)6-Phe-tRNA.",

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N2 - The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serinen (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)6-Phe-tRNA.

AB - The synthesis of l-serine and l-tyrosine containing oligopeptidyl-tRNA is described. The method involves a reaction between N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-serinen (n = 1-2) or N-o-nitrophenylsulfenyl-(l-Tyr)n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3. In the case of N-protected carboxyl-activated l-Ser-L-Ser, the acylation reaction was repeated three times resulting in the formation of (l-Ser)6-Phe-tRNA.

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Peptidyl transfer RNA. X. The chemical synthesis of peptidyl transfer RNA containing hydroxy amino acids

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