Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA

Y. Lapidot*, D. Elat, S. Rappoport, N. de Groot

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[14C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [14C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.

Original languageEnglish
Pages (from-to)559-563
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume38
Issue number4
DOIs
StatePublished - 20 Feb 1970

Fingerprint

Dive into the research topics of 'Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA'. Together they form a unique fingerprint.

Cite this