Abstract
The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[14C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [14C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.
Original language | English |
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Pages (from-to) | 559-563 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 38 |
Issue number | 4 |
DOIs | |
State | Published - 20 Feb 1970 |